Freeze-thaw

Proacrosin activation mechanisms in capacitated and frozen-thawed boar spermatozoa

J. J. Cui, Tian, Y., Liu, Y., Huang, H. L., and Jin, Y., Proacrosin activation mechanisms in capacitated and frozen-thawed boar spermatozoa, vol. 13, pp. 9915-9920, 2014.

The main objective of the current study was to explore the different activation mechanisms of capacitation and freeze-thawed spermatozoa. Using SDS-PAGE and Western blotting, the conversion process of boar proacrosin during freeze-thawing and capacitation of spermatozoa was analyzed. The results revealed that capacitated spermatozoa exhibited a greater fluorescence area than that of the freeze-thawed spermatozoa, which were smaller than those of the fresh group.

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