Formalin-fixed tissue

Improved protocol for extracting genomic DNA from frozen formalin-fixed tissue resulting in high-quality whole mtDNA

H. W. Wang, Xu, Y., Zhang, H. F., Zeng, Y. J., Ren, L., Miao, Y. L., Luo, H. Y., Wang, K. H., Wang, H. W., Xu, Y., Zhang, H. F., Zeng, Y. J., Ren, L., Miao, Y. L., Luo, H. Y., and Wang, K. H., Improved protocol for extracting genomic DNA from frozen formalin-fixed tissue resulting in high-quality whole mtDNA, vol. 15, p. -, 2016.

Formalin fixation and paraffin embedding is widely used for convenient and long-term storage of tumor tissue and precious sources to perform genetic studies. However, DNA fragmentation is one of the major flaws of genomic DNA isolation from formalin fixation tissues, which limits its further usage. Here, we present an improved method for isolating high-quality genomic DNA from formalin fixation tissue. We obtained high-quality genomic DNA of more than 20 kb from samples frozen for more than 2 years.

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