Forensic entomology, the study of insects associated with forensic procedures, aims to gather information and traces that can assist an investigation, demonstrating the importance of both science and legal processes (Thyssen, 2011). Among the various uses of insects in investigations, entomogenetics combines forensic genetic techniques and the versatility of entomology.
Sarcophagidae, or flesh flies, are of great importance in forensic entomology, but their effective application requires precise taxonomic identification, which relies almost exclusively on characteristics of the male genitalia. Given that female flies and larvae are most abundant in animal carcasses or on corpses, precise morphological identification can be difficult; therefore, DNA sequencing can be an additional tool for use in taxonomic identification.
Investigations of any type of crime invariably starts at the crime scene by collecting evidence. Thus, the purpose of this research was to collect and analyze an entomological trace from an environment that is similar to those of indoor crime scenes. Hematophagous mosquitoes were collected from two residential units; saliva of volunteers that were residents in the units was also collected for genetic analysis as reference samples.