Fluorescence detection

Construction of a primary DNA fingerprint database for cotton cultivars

Y. C. Zhang, Kuang, M., Yang, W. H., Xu, H. X., Zhou, D. Y., Wang, Y. Q., Feng, X. A., Su, C., and Wang, F., Construction of a primary DNA fingerprint database for cotton cultivars, vol. 12, pp. 1897-1906, 2013.

Forty core primers were used to construct a DNA fingerprint database of 132 cotton species based on multiplex fluorescence detection technology. A high first successful ratio of 99.04% was demonstrated with tetraplex polymerase chain reaction. Forty primer pairs amplified a total of 262 genotypes among 132 species, with an average of 6.55 per primer and values of polymorphism information content varying from 0.340 to 0.882. Conflicting DNA homozygous ratios were found in various species.

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