Flow cytometry

Inducing autotetraploids in cassava using oryzalin and colchicine and their in vitro morphophysiological effects

deJ. da S. M. de Carvalho, Gomes, V. B., A. Souza, daS., Aud, F. F., Santos-Serejo, J. A., Oliveira, E. J., M. de Carvalho, deJ. da S., Gomes, V. B., A. Souza, daS., Aud, F. F., Santos-Serejo, J. A., and Oliveira, E. J., Inducing autotetraploids in cassava using oryzalin and colchicine and their in vitro morphophysiological effects, vol. 15, p. -, 2016.

Polyploid induction has been used for plant breeding to produce bigger and more robust plants than diploid types. The present study aimed to develop a methodology for in vitro induction of polyploidy in cassava. Apical and lateral microcuttings from the BRS Formosa variety were treated with six oryzalin concentrations for 24 and 48 h. The same methodology was used for colchicine with different concentrations. After 45 days of cultivation and an additional 45 days of subculture, the viability of the explants was assessed and plant acclimatization was performed.

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