Fibroblasts

Tetrandrine induces microRNA differential expression in human hypertrophic scar fibroblasts in vitro

P. Ning, Peng, Y., Liu, D. W., Hu, Y. H., Liu, Y., Liu, D. M., Ning, P., Peng, Y., Liu, D. W., Hu, Y. H., Liu, Y., and Liu, D. M., Tetrandrine induces microRNA differential expression in human hypertrophic scar fibroblasts in vitro, vol. 15, p. -, 2016.

MicroRNAs (miRNAs) have recently been shown to play a role in normal wound healing process. miRNAs may be linked to pathologic wound healing and closely related to the formation of hypertrophic scars. This study aimed to explore the effects of tetrandrine on the miRNA expression profile in human hypertrophic scar fibroblasts (HSFs) in vitro. HSFs were randomly divided into two groups: the tetrandrine treatment group and the control group. The experimental and control groups were collected and analyzed by miRNA array after a 48-h culture.

Effects of eplerenone on the activation of matrix metalloproteinase-2 stimulated by high glucose and interleukin-1β in human cardiac fibroblasts

J. F. Chi, Uzui, H., Guo, H. Y., Ueda, T., and Lee, J. D., Effects of eplerenone on the activation of matrix metalloproteinase-2 stimulated by high glucose and interleukin-1β in human cardiac fibroblasts, vol. 13, pp. 4845-4855, 2014.

The aim of this study was to determine the influence of high glucose (HG) and interleukin (IL)-1β on human cardiac fibroblast (HCF) functions, and to evaluate the effects of eplerenone in these responses. HCFs were cultured in normal or HG media in the absence or presence of IL-1β and/or eplerenone. We assessed matrix metalloproteinase-2 (MMP-2) activity in the supernatant by in-gel zymography, and determined mRNA expression levels of MMP-2 and tissue inhibitor of metalloproteinase-2 (TIMP-2) by reverse transcription-polymerase chain reaction.

Effectiveness of liposomes to transfect livestock fibroblasts

R. R. Oliveira, de Carvalho, D. M., Lisauskas, S., Mello, E., Vianna, G. R., Dode, M. A. N., Rumpf, R., Aragão, F. J. L., Rech, E. L., and Rech, E. L., Effectiveness of liposomes to transfect livestock fibroblasts, vol. 4, pp. 185-196, 2005.

The development of an efficient transfection system in livestock cells is an important step towards investigating gene transfer and the functioning and production of transgenic animals. Important factors involved in cationic liposome mediated gene transfer were evaluated through in vitro transfection of bovine, caprine and ovine fibroblast cells.

Development of bovine embryos reconstructed by nuclear transfer of transfected and non-transfected adult fibroblast cells

L. T. Iguma, Iguma, L. T., Melo, E. O., Franco, M. M., Pivato, I., Vianna, G. R., Sousa, R. V., Dode, M. A. N., Aragão, F. J. L., Rech, E. L., Rech, E. L., Rumpf, R., and Lisauskas, S. F. C., Development of bovine embryos reconstructed by nuclear transfer of transfected and non-transfected adult fibroblast cells, vol. 4, pp. 55-66, 2005.

An association of two techniques, nuclear transfer (NT), and transfection of somatic animal cells, has numerous potential applications and considerable impact, mainly in agriculture, medicine, pharmacy, and fundamental biology. In addition, somatic cell nuclear transfer is the most efficient alternative to produce large transgenic animals. We compared in vitro and in vivo developmental capacities of NT using fibroblast cells isolated from a 14-month-old cloned Simmental heifer (FCE) vs the same line transfected with a plasmid containing neomycin-resistant genes (TFCE).

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