False-positive exclusion

Single- and double-SSR primer combined analyses in rice

J. Ma, Guan, S. C., Zhang, Z., and Wang, P. W., Single- and double-SSR primer combined analyses in rice, vol. 11, pp. 1032-1038, 2012.

Polymerase chain reaction (PCR) is the foundation of SSR molecular marker technology. We used sib rice varieties J518, XD1 and SD23 as experimental materials, selecting 30 pairs of SSR primers, including RM127, RM337 and RM5172, covering the rice genome, and performed single- and double-SSR primer combined analyses. We found that under the same PCR system and conditions, a single primer of the SSR primer pairs could amplify the same fragments as double primers do.

Single-primer PCR correction: a strategy for false-positive exclusion

J. Ma, Wang, P. W., Yao, D., Wang, Y. P., Yan, W., and Guan, S. C., Single-primer PCR correction: a strategy for false-positive exclusion, vol. 10, pp. 150-159, 2011.

Polymerase chain reaction (PCR) technology plays an important role in molecular biology research, but false-positive and nonspecific PCR amplification have plagued many researchers. Currently, research on the optimization of the PCR system focuses on double-primer-based PCR products.

Problems with and a system to eliminate single-primer PCR product contamination in simple sequence repeat molecular marker-assisted selection in soybean

J. Ma, Guan, S. C., Yao, D., Wei, Y. F., and Wang, P. W., Problems with and a system to eliminate single-primer PCR product contamination in simple sequence repeat molecular marker-assisted selection in soybean, vol. 10, pp. 1659-1668, 2011.

Polymerase chain reaction (PCR) provides a foundation for simple sequence repeat molecular marker-assisted selection (SSR MAS) in soybean. This PCR system and its various conditions have been optimized by many researchers. However, current research on the optimization of the PCR system focuses on double-primer PCR products.

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