F-PCR-RFLP

Impacts of single nucleotide polymorphisms and haplotypes in the bovine Dapper1 gene on body weight

J. Wang, Wang, C., Gao, Y., Lan, X. - Y., Lei, C. - Z., Wang, J. - Q., and Chen, H., Impacts of single nucleotide polymorphisms and haplotypes in the bovine Dapper1 gene on body weight, vol. 12, pp. 1254-1268, 2013.

The Dapper1 protein plays important role in multiple developmental processes via negative modulation of the Wnt signaling pathway. We detected variations in Dapper1 in 1185 individuals from 5 Chinese cattle breeds and determined their effects on bovine body weight. Two silent mutations (g.8344C>T and g.8428C>T) in exon 6 along with two substitutions (g.10513A>G and g.10765C>G) in the 3'-untranslated region were detected with DNA pool sequencing and forced polymerase chain reaction-restriction fragment length polymorphism.

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