EZH2

Expression and clinical implications of enhancer of Zeste homolog 2 and p53 protein in squamous cell carcinoma and precancerous lesions in the cervix

H. M. Zhang, Chen, S. Q., Yao, S. Z., Zhang, H. M., Chen, S. Q., and Yao, S. Z., Expression and clinical implications of enhancer of Zeste homolog 2 and p53 protein in squamous cell carcinoma and precancerous lesions in the cervix, vol. 15, p. -, 2016.

We investigated the expression and clinical implications of enhancer of Zeste homolog 2 (EZH2) and p53 protein in cervical squamous cell carcinoma (SCC) and precancerous lesions. EZH2 and p53 expressions in SCC (168), cervical intraepithelial neoplasia (CIN)-I (19), CIN-II (35), and normal tissues (30) were detected by streptavidin-peroxidase-conjugation. The correlation between co-expression of EZH2 and p53 protein and the clinic pathological features and prognosis of SCC were discussed.

EZH2 suppresses hepatocellular differentiation of mouse bone marrow mesenchymal stem cells

T. Lu, Sun, H., Lv, J., Yang, M. F., Zhang, F., Qian, Y., and Dong, X. J., EZH2 suppresses hepatocellular differentiation of mouse bone marrow mesenchymal stem cells, vol. 13, pp. 2231-2239, 2014.

Our previous studies have indicated that mouse bone marrow mesenchymal stem cells (mBMMSCs) have potential to differentiate into hepatocytes with high efficiency. Our study aimed to evaluate the role of the mouse histone methyltransferase enhancer of zeste homolog 2 gene (EZH2) in the hepatocellular differentiation of mBMMSCs. The mBMMSCs isolated from femurs and tibias were cultured in Iscove's modified Eagle's medium (IMEM) supplemented with 10% fetal bovine serum.

H3K27me3 may be associated with Oct4 and Sox2 in mouse preimplantation embryos

F. - R. Wu, Zhang, Y., Ding, B., Lei, X. - H., Huang, J. - C., Wang, C. - H., Liu, Y., Wang, R., and Li, W. - Y., H3K27me3 may be associated with Oct4 and Sox2 in mouse preimplantation embryos, vol. 13, pp. 10121-10129, 2014.

As a core member of polycomb repressive complex 2, the transcription and enzyme activity of enhancer of zeste homolog 2 (Ezh2) is directly involved in the trimethylation of lysine 27 on histone H3. In this study, the fluorescence intensity of H3K27me3 in mouse in vivo morulae and blastocysts was compared by indirect immunofluorescence staining. We found that demethylation of H3K27me3 occurred during the blastocyst stage. Real-time polymerase chain reaction was performed to investigate Ezh2 expression in oocytes and in preimplantation embryos.

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