Extraction efficiency

Comparison of extraction methods of total microbial DNA from freshwater

P. Li, Yang, S. F., Lv, B. B., Zhao, K., Lin, M. F., Zhou, S., Song, X., and Tang, X. M., Comparison of extraction methods of total microbial DNA from freshwater, vol. 14, pp. 730-738, 2015.

The demand for molecular analysis of aquatic microbial communities in freshwater has highlighted the need for efficient methods of DNA extraction. The centrifugation method and filtration-membrane method are 2 widely used methods for extracting DNA. The objective of this study was to compare the extraction efficiency of 3 methods, including the centrifugation method, filtration-membrane method, and modified filtration-membrane method, by evaluating the quantity and purity of DNA extracts obtained from water.

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