Extraction

Evaluation of methods of DNA extraction from Staphylococcus aureus in milk for use in real-time PCR

A. G. Dibbern, Botaro, B. G., Viziack, M. P., Silva, L. F. P., and Santos, M. V., Evaluation of methods of DNA extraction from Staphylococcus aureus in milk for use in real-time PCR, vol. 14, pp. 227-233, 2015.

The aim of this study was to evaluate the repeatability and performance of 4 methods of extracting DNA from Staphylococcus aureus (SAU) and the gene encoding bovine mitochondrial cytochrome B (BMCB) in milk samples from cows with subclinical mastitis for use in amplification by real-time polymerase chain reaction. Two milk samples were obtained from cows naturally infected with S. aureus and subjected to the following extraction methods: Qiagen DNA extraction kit; Axyprep DNA extraction kit; in silica column boil and in silica column method.

Optimization of the procedure for extracting nucleic acids from aloe

J. Li, Zhang, Y. S., Liao, C. M., and Liu, X. H., Optimization of the procedure for extracting nucleic acids from aloe, vol. 13, pp. 276-282, 2014.

Aloe, an important folk herbal drug, includes abundant polysaccharides and secondary metabolites, which make it difficult to isolate high-quality DNA or RNA. In this paper, one and two improved methods were used to isolate the genomic DNA and RNA from the leaf of aloe, respectively. The obtained samples presented good quality and integrity; thus, they could be further used for many downstream molecular experiments. These reported protocols for DNA and RNA extraction offered a valuable reference for other related studies.

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