Expression system

Identification of BPI protein produced in different expression system and its association with Escherichia coli F18 susceptibility

Z. C. Wu, Liu, Y., Dong, W. H., Sun, S. Y., Zhu, G. Q., Wu, S. L., and Bao, W. B., Identification of BPI protein produced in different expression system and its association with Escherichia coli F18 susceptibility, vol. 14, pp. 1111-1123, 2015.

The super antibiotic bactericidal/permeability-increasing (BPI) protein is a member of a new generation of proteins that have been implicated as endotoxin-neutralizing agents. In this study, recom­binant porcine BPI protein was obtained by generating porcine BPI encoding prokaryotic, eukaryotic, and yeast expression vectors. Recombinant protein expression was detected in yeast GS115, Escherichia coli, and 293-6E cells by gel electrophoresis and Western blotting.

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