Expression analysis

Cloning and expression analysis of cysteine protease gene (MwCP) in Agropyron mongolicum Keng

T. G. B. Y. Ao, Lang, M. L., Li, Y. Q., Zhao, Y., Wang, L. C., Yang, X. J., Ao, T. G. B. Y., Lang, M. L., Li, Y. Q., Zhao, Y., Wang, L. C., Yang, X. J., Ao, T. G. B. Y., Lang, M. L., Li, Y. Q., Zhao, Y., Wang, L. C., and Yang, X. J., “Cloning and expression analysis of cysteine protease gene (MwCP) in Agropyron mongolicum Keng”, vol. 15, p. -, 2016.

In this study, a cysteine protease gene (MwCP) from Agropyron mongolicum Keng was isolated using RACE. Sequence analysis indicated that MwCP was 1473 bp, and it contained a 1134-bp open reading frame, which encoded 377 amino acids with a 24-amino acid N-terminal signal peptide. The results indicated that the MwCP protein was a new member of the papain C1A family, and it was predicted to be an extracellular, secretory stable hydrophilic protein.

Cloning and characterization of the SERK1 gene in triploid Pingyi Tiancha [Malus hupehensis (Pamp.) Rehd. var. pingyiensis Jiang] and a tetraploid hybrid strain

L. J. Zhang, Dong, W. X., Guo, S. M., Wang, Y. X., Wang, A. D., and Lu, X. J., “Cloning and characterization of the SERK1 gene in triploid Pingyi Tiancha [Malus hupehensis (Pamp.) Rehd. var. pingyiensis Jiang] and a tetraploid hybrid strain”, vol. 14, pp. 14576-14586, 2015.

This study aims to explore the roles of somatic embryogenesis receptor-like kinase (SERK) in Malus hupehensis (Pingyi Tiancha). The full-length sequences of SERK1 in triploid Pingyi Tiancha (3n) and a tetraploid hybrid strain 33# (4n) were cloned, sequenced, and designated as MhSERK1 and MhdSERK1, respectively.

Comprehensive identification and expression analysis of Hsp90s gene family in Solanum lycopersicum

W. S. Zai, Miao, L. X., Xiong, Z. L., Zhang, H. L., Ma, Y. R., Li, Y. L., Chen, Y. B., and Ye, S. G., “Comprehensive identification and expression analysis of Hsp90s gene family in Solanum lycopersicum”, vol. 14, pp. 7811-7820, 2015.

Heat shock protein 90 (Hsp90) is a protein produced by plants in response to adverse environmental stresses. In this study, we identified and analyzed Hsp90 gene family members using a bioinformatic method based on genomic data from tomato (Solanum lycopersicum L.). The results illustrated that tomato contains at least 7 Hsp90 genes distributed on 6 chromosomes; protein lengths ranged from 267-794 amino acids. Intron numbers ranged from 2-19 in the genes.

Molecular cloning and expression analysis of a novel BCCP subunit gene from Aleurites moluccana

W. Y. Xuan, Zhang, Y., Liu, Z. Q., Feng, D., and Luo, M. Y., “Molecular cloning and expression analysis of a novel BCCP subunit gene from Aleurites moluccana”, vol. 14, pp. 9922-9931, 2015.

Aleurites moluccana L. is grown as a roadside tree in southern China and the oil content of its seed is higher than other oil plants, such as Jatropha curcas and Camellia oleifera. A. moluccana is considered a promising energy plant because its seed oil could be used to produce biodiesel and bio-jet fuel. In addition, the bark, leaves, and kernels of A. moluccana have various medical and commercial uses. Here, a novel gene coding the biotin carboxyl carrier protein subunit (BCCP) was cloned from A. moluccana L.

Subscribe to Expression analysis