Expression analysis

Cloning and expression analysis of pepper chlorophyll catabolite reductase gene CaRCCR

H. - J. Xiao, Jin, J. - H., Chai, W. - G., and Gong, Z. - H., Cloning and expression analysis of pepper chlorophyll catabolite reductase gene CaRCCR, vol. 14, pp. 368-379, 2015.

Opening the porphyrin macrocycle of pheophorbide a and forming the primary fluorescent chlorophyll catabolites are key steps in the chlorophyll catabolism pathway. These steps are catalyzed by pheophorbide a oxygenase and red chlorophyll catabolite reductase (RCCR). In this study, a novel RCCR gene, CaRCCR, was isolated from the pepper (Capsicum annuum L.). The full-length CaRCCR complementary DNA is comprised of 1173 bp, contains an open reading frame of 945 bp, and encodes a 314-amino acid protein.

Genome-wide identification and expression analysis of the CPP-like gene family in soybean

L. Zhang, Zhao, H. K., Wang, Y. M., Yuan, C. P., Zhang, Y. Y., Li, H. Y., Yan, X. F., Li, Q. Y., and Dong, Y. S., Genome-wide identification and expression analysis of the CPP-like gene family in soybean, vol. 14, pp. 1260-1268, 2015.

Cysteine-rich polycomb-like protein (CPP-like) genes are a group of transcription factors with highly conserved cysteine-rich domains and are widely distributed in animals and plants, but do not present in yeast. Previous studies have shown that members of this family play important roles in the development of reproductive tissue and in the control of cell division in plants. In this study, whole genome identification of soybean CPP transcription factors was performed using bioinformatic methods.

Molecular cloning, characterization and expression analysis of a Doublesex gene from Daphnia carinata (Crustacea: Cladocera) during different reproductive stages

M. Q. Zhang, Ma, C. A., Lv, W. W., Huang, Y. H., Wang, D. L., and Zhao, Y. L., Molecular cloning, characterization and expression analysis of a Doublesex gene from Daphnia carinata (Crustacea: Cladocera) during different reproductive stages, vol. 14, pp. 5930-5842, 2015.

To better understand the reproductive transformation mechanism of Daphnia carinata, a Doublesex (Dsx) gene was cloned based on rapid amplification of cDNA ends (RACE), and was designated DapcaDsx2. Next, we compared similarities and assumed homology based on deduced amino acid sequences. It showed 97.52, 87.94, and 85.11% identity to orthologous genes in D. magna, D. pulex, and D. galeata respectively.

Cloning and expression of an APETALA1-like gene from Nelumbo nucifera

D. Z. Kong, Shen, X. Y., Guo, B., Dong, J. X., Li, Y. H., and Liu, Y. P., Cloning and expression of an APETALA1-like gene from Nelumbo nucifera, vol. 14, pp. 6819-6829, 2015.

The objective of this study was to clone the full-length cDNA of the APETALA1 (AP1) gene from lotus and analyze its sequence and expression pattern. The full-length cDNA sequence of the NnAP1 gene was amplified from the petals of Nelumbo nucifera ‘Hongxia’ using RT-PCR and rapid amplification of cDNA ends. Bioinformatic methods were used to analyze the sequence characteristics of the gene.

Cloning and expression analysis of the Lonicera japonica Thunb. chlorogenic acid synthetase gene (LjCCoAOMT1) in rice

X. - H. Jiang, She, C. - W., Zhu, Y. - H., and Liu, X. - M., Cloning and expression analysis of the Lonicera japonica Thunb. chlorogenic acid synthetase gene (LjCCoAOMT1) in rice, vol. 13, pp. 2166-2176, 2014.

Complete coding DNA sequences of a closely related chlorogenic acid synthetase gene (LjCCoAOMT1) were isolated from Lonicera japonica Thunb. by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). LjCCoAOMT1 was subsequently overexpressed in Escherichia coli and a 25-kD protein was detected by electrophoresis and western blot analysis. High-performance liquid chromatography (HPLC) analysis showed that recombinant LjCCoAOMT1 methylates the caffeic acid substrate to generate ferulic acid.

Expression pattern of the zona pellucida 3 (ZP3) gene during ovarian development and the location of ZP3 protein in oocytes in a natural, wild triploid crucian carp mutant, Carassius auratus var. Pingxiangnensis

J. W. Shi, Sheng, J. Q., Peng, K., Wang, J. H., Yi, W. J., Wu, H. J., Gu, Q., and Hong, Y. J., Expression pattern of the zona pellucida 3 (ZP3) gene during ovarian development and the location of ZP3 protein in oocytes in a natural, wild triploid crucian carp mutant, Carassius auratus var. Pingxiangnensis, vol. 12, pp. 5640-5650, 2013.

Carassius auratus var. Pingxiangnensis (designated CaP), distributed in the Pingxiang region of Jiangxi Province, China, is a natural, wild triploid crucian carp mutant that has two reproductive development modes: gynogenesis and bisexual reproduction. Little information is available about the expression pattern of the zona pellucida 3 (ZP3) gene during ovarian development and the location of the ZP3 protein in oocytes of this fish. In this study, we obtained the full-length cDNA of ZP3 (CaP_ZP3).

Cloning and expression analysis of the 37-kDa laminin receptor precursor gene from Hyriopsis cumingii

X. Z. Chang, Li, J. L., Bai, Z. Y., and Li, X. L., Cloning and expression analysis of the 37-kDa laminin receptor precursor gene from Hyriopsis cumingii, vol. 12, pp. 6130-6139, 2013.

Hyriopsis cumingii is an economically important freshwater pearl mussel with high pearl quality that is endemic in China. Investigation of genes relevant to shell formation is important for increased pearl output. The substances that form mollusk shells are secreted by epithelial cells in the mantle, the proliferation of which influences secretion ability. This study focused on the proliferation-related 37-kDa laminin receptor precursor (37LRP) of H. cumingii. The full-length cDNA (1133 bp) encoding this 300-amino acid protein was cloned from the mantle.

Subscribe to Expression analysis