Expressed sequence tags

Development of polymorphic SSR markers in the razor clam (Sinonovacula constricta) and cross-species amplification

Y. H. Dong, Yao, H. H., Sun, C. S., Lv, D. M., Li, M. Q., Lin, Z. H., Dong, Y. H., Yao, H. H., Sun, C. S., Lv, D. M., Li, M. Q., Lin, Z. H., Dong, Y. H., Yao, H. H., Sun, C. S., Lv, D. M., Li, M. Q., and Lin, Z. H., Development of polymorphic SSR markers in the razor clam (Sinonovacula constricta) and cross-species amplification, vol. 15, p. -, 2016.

Next-generation sequencing provides large-scale sequencing data with relative ease and at a reasonable cost, making it possible to identify a large amount of SSR markers in a timely and cost-effective manner. On the basis of the transcriptome database of Sinonovacula constricta obtained by Illumina/Solexa pyrosequencing, 60 polymorphic SSR markers were developed and characterized in 30 individuals. The number of alleles per polymorphic locus ranged from 2 to 7 with an average of 3.75 alleles.

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