Development of polymorphic SSR markers in the razor clam (Sinonovacula constricta) and cross-species amplification
, “Development of polymorphic SSR markers in the razor clam (Sinonovacula constricta) and cross-species amplification”, vol. 15, p. -, 2016.
Next-generation sequencing provides large-scale sequencing data with relative ease and at a reasonable cost, making it possible to identify a large amount of SSR markers in a timely and cost-effective manner. On the basis of the transcriptome database of Sinonovacula constricta obtained by Illumina/Solexa pyrosequencing, 60 polymorphic SSR markers were developed and characterized in 30 individuals. The number of alleles per polymorphic locus ranged from 2 to 7 with an average of 3.75 alleles.
