Escherichia coli F18

Identification of BPI protein produced in different expression system and its association with Escherichia coli F18 susceptibility

Z. C. Wu, Liu, Y., Dong, W. H., Sun, S. Y., Zhu, G. Q., Wu, S. L., and Bao, W. B., Identification of BPI protein produced in different expression system and its association with Escherichia coli F18 susceptibility, vol. 14, pp. 1111-1123, 2015.

The super antibiotic bactericidal/permeability-increasing (BPI) protein is a member of a new generation of proteins that have been implicated as endotoxin-neutralizing agents. In this study, recom­binant porcine BPI protein was obtained by generating porcine BPI encoding prokaryotic, eukaryotic, and yeast expression vectors. Recombinant protein expression was detected in yeast GS115, Escherichia coli, and 293-6E cells by gel electrophoresis and Western blotting.

Dynamic changes in TAP1 expression levels in newborn to weaning piglets, and its association with Escherichia coli F18 resistance

J. Wang, Liu, Y., Dong, W. H., Huo, Y. J., Huang, X. G., Wu, S. L., and Bao, W. B., Dynamic changes in TAP1 expression levels in newborn to weaning piglets, and its association with Escherichia coli F18 resistance, vol. 13, pp. 3686-3692, 2014.

The transporter associated with antigen processing (TAP) transports peptides from the cytosol into the endoplasmic reticulum for subsequent loading onto the major histocompatibility complex (MHC) class I molecules. This study showed the dynamic changes in the TAP1 expression level in newborn to weaning piglets.

TLR4 gene expression in pig populations and its association with resistance to Escherichia coli F18

J. Wang, Pan, Z. Y., Zheng, X. R., Wu, Z. C., Su, X. M., Zhu, G. Q., Huang, X. G., Wu, S. L., and Bao, W. B., TLR4 gene expression in pig populations and its association with resistance to Escherichia coli F18, vol. 12, pp. 2625-2632, 2013.

TLR4 is the main recognition receptor of bacterial lipopolysaccharides, which play an important role in innate and adaptive immunity. We used real-time PCR to analyze the tissue expression profile and differential expression of TLR4 in 4 pig populations (Escherichia coli F18-resistant Sutai, E. coli F18-sensitive Sutai, Large White, Meishan), in order to determine the role that the TLR4 gene plays in resistance to E. coli F18.

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