Enriched library

Isolation and characterization of microsatellite loci from the tick Amblyomma aureolatum (Acari: Ixodidae)

M. Ogrzewalska, Bajay, M. M., Schwarcz, K., Bajay, S. K., Telles, M. P. C., Pinheiro, J. B., Zucchi, M. I., Pinter, A., and Labruna, M. B., “Isolation and characterization of microsatellite loci from the tick Amblyomma aureolatum (Acari: Ixodidae)”, vol. 13, pp. 9622-9627, 2014.

Amblyomma aureolatum (Pallas) is the main vector of the bacterium Rickettsia rickettsii, the etiological agent of Brazilian spotted fever. This disease is the most lethal human spotted fever rickettsiosis in the world. Microsatellite loci were isolated from a dinucleotide-enriched library produced from A. aureolatum sampled in Southeastern Brazil. Eight polymorphic microsatellites were further characterized among 38 individuals sampled from São Paulo metropolitan region.

Isolation and characterization of polymorphic microsatellite loci in the ridgetail white prawn Exopalaemon carinicauda

S. W. Jia, Liu, P., Li, J., Li, J. T., and Pan, L. Q., “Isolation and characterization of polymorphic microsatellite loci in the ridgetail white prawn Exopalaemon carinicauda”, vol. 12, pp. 2816-2820, 2013.

The ridgetail white prawn Exopalaemon carinicauda is one of the major economic shrimp species cultured in China. In this study, 30 microsatellite loci were isolated and characterized for the ridgetail white prawn E. carinicauda using a microsatellite-enriched library. Polymorphisms were tested in 30 individuals from a single wild population. The number of alleles at each locus ranged from 2 to 14. The observed and expected heterozygosities varied from 0.1000 to 0.8000 and from 0.2299 to 0.9228, respectively. The PIC value ranged from 0.2002 to 0.8939.

Isolation and characterization of 16 novel microsatellite loci in two inbred strains of the Chinese hamster (Cricetulus griseus)

G. - H. Song, Geng, J. - N., Jia, R. - Y., Yue, W. - B., Liu, T. - F., and Hu, S. - N., “Isolation and characterization of 16 novel microsatellite loci in two inbred strains of the Chinese hamster (Cricetulus griseus)”, vol. 10, pp. 2245-2256, 2011.

The Shanyi inbred A and E strains of the Chinese hamster are widely used in biomedical research, but detailed genetic characterization has been lacking. We developed microsatellite markers that could be used for genetic diversity analysis and linkage map construction. We isolated and characterized 16 novel microsatellite loci from a microsatellite-enriched genomic DNA library. These loci were genotyped in 48 animals from the two strains, and the polymorphic information content was determined.

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