Endogenous genes

Reference genes for quantitative real-time polymerase chain reaction studies in soybean plants under hypoxic conditions

T. J. Nakayama, Rodrigues, F. A., Neumaier, N., Marcelino-Guimarães, F. C., Farias, J. R. B., de Oliveira, M. C. N., Borém, A., de Oliveira, A. C. B., Emygdio, B. M., and Nepomuceno, A. L., Reference genes for quantitative real-time polymerase chain reaction studies in soybean plants under hypoxic conditions, vol. 13, pp. 860-871, 2014.

Quantitative real-time polymerase chain reaction (RT-qPCR) is a powerful tool used to measure gene expression. However, because of its high sensitivity, the method is strongly influenced by the quality and concentration of the template cDNA and by the amplification efficiency. Relative quantification is an effective strategy for correcting random and systematic errors by using the expression level of reference gene(s) to normalize the expression level of the genes of interest.

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