ELISA

Analysis of tacrolimus blood concentrations in renal transplant patients

C. Y. Wang, Xu, X., Li, M. C., Li, Q., and Ji, S. G., “Analysis of tacrolimus blood concentrations in renal transplant patients”, vol. 14, pp. 3791-3797, 2015.

This study aimed to 1) analyze the results of tacrolimus blood concentration monitored in patients after renal transplantation, 2) observe and establish an optimal therapeutic window for patients, and 3) provide evidence for the clinical and rational use of drugs. Tacrolimus blood concentration was determined by enzyme-linked immunosorbent assay. A total of 1824 cases were obtained from the monitoring of 74 patients after renal transplantation. These cases were then retrospectively analyzed.

Non-invasive determination of the immune physiological state of reindeer (Rangifer tarandus) in the Greater Khingan Mountains, China

Y. J. Yin, Nie, C. Y., Liu, W. S., Zou, Q., Zhai, J. C., Han, H. S., and Li, H. P., “Non-invasive determination of the immune physiological state of reindeer (Rangifer tarandus) in the Greater Khingan Mountains, China”, vol. 14, pp. 6664-6673, 2015.

Immunoglobulin and cortisol levels are good indicators of well-being and living status in animals. In this study, the concentrations of fecal immunoglobulins A ([IgAF]), G ([IgGF]), and M ([IgMF]), and cortisol ([cortisolF]) were examined by enzyme-linked immunosorbent assay in reindeer of the Greater Khingan Mountains of Inner Mongolia, China.

FlgN plays important roles in the adhesion of Aeromonas hydrophila to host mucus

X. Jiang, Qin, Y. X., Lin, G. F., Huang, L., Huang, B., Huang, W. S., and Yan, Q. P., “FlgN plays important roles in the adhesion of Aeromonas hydrophila to host mucus”, vol. 14, pp. 6376-6386, 2015.

Adhesion to the host mucus is a crucial step in the early infection stage of pathogenic bacteria. To investigate the mechanisms of the adhesion of Aeromonas hydrophila to its host mucus, a mutant library was constructed using the mini-Tn10 transposon mutagenesis system. Of 276 individual colonies, the mutant strain with the most attenuated adhesion ability in this study was screened out and designated A77.

Expression and serological diagnosis of Mycobacterium tuberculosis CFP-10 and Rv2626c proteins

Z. Y. Zhu, Zhang, D., Wang, H. B., Xiao, J. Z., Qiu, Y. F., Yan, L., Chen, D., Liu, A. G., and Yang, X., “Expression and serological diagnosis of Mycobacterium tuberculosis CFP-10 and Rv2626c proteins”, vol. 13, pp. 7398-7406, 2014.

We constructed a Mycobacterium tuberculosis vector expressing CFP-10 and Rv2626c to examine the expression of these proteins in Escherichia coli as well as their immunoreactivity. The CFP-10 and Rv2626c genes were amplified from tuberculosis H37Rv genomic DNA using polymerase chain reaction. They were ligated into the expression vector PET30a and expressed in E. coli. Histidine tag nickel column chromatography was used to purify the recombinant protein. An enzyme-linked immunosorbent assay (ELISA) was used for detection. In our E.

Piperine inhibits cytokine production by human peripheral blood mononuclear cells

S. Chuchawankul, Khorana, N., and Poovorawan, Y., “Piperine inhibits cytokine production by human peripheral blood mononuclear cells”, vol. 11, pp. 617-627, 2012.

Piperine, an amide isolated from Piper species (Piperaceae), has been reported to exhibit central nervous system depression, anti-pyretic and anti-inflammatory activity. Immunomodulatory and anti-tumor activity of piperine has been demonstrated in mouse carcinomas. However, there is little information available concerning the effect of piperine on humans. We evaluated the immunopharmacological activity of this compound in human immune cells. Human peripheral blood mononuclear cells (PBMCs) were exposed to piperine, and cell proliferation was determined by the MTS assay.

Expression of the luteinizing hormone receptor (LHR) gene in ovine non-gonadal tissues during estrous cycle

L. H. Wang, Zhang, W., Gao, Q. X., and Wang, F., “Expression of the luteinizing hormone receptor (LHR) gene in ovine non-gonadal tissues during estrous cycle”, vol. 11, pp. 3766-3780, 2012.

Luteinizing hormone (LH) is an important glycoprotein hormone that regulates gonadal function in mammals and in turn regulates physiological status changes during the estrous cycle. The function of LH is mediated by luteinizing hormone receptor (LHR).

Fast preparation of a polyclonal antibody against chicken protocadherin 1

Y. H. Li, Zhao, X. H., Li, M., Yang, C. Q., Wang, L., and Lin, J. T., “Fast preparation of a polyclonal antibody against chicken protocadherin 1”, vol. 12, pp. 2156-2166, 2013.

Protocadherins constitute a large family belonging to the cadherin superfamily; they function in various tissues of a wide variety of multicellular organisms. However, their functions and expression modes are still unknown in many of these species and tissues. We developed a fast and low-cost method to produce polyclonal antibody against chicken protocadherin 1 (Pcdh1) that could be used in assays for immunological assessment of protein expression levels of chicken Pcdh1.

Antibody-based detection of alkaline phosphatase in lepidopteran insects (Lepidoptera: Noctuidae)

R. A. Khan, Rashid, M., Wang, D., and Zhang, Y. L., “Antibody-based detection of alkaline phosphatase in lepidopteran insects (Lepidoptera: Noctuidae)”, vol. 12, pp. 4371-4382, 2013.

Alkaline phosphatase (ALP) of Helicoverpa armigera Hub. (Lepidoptera; Noctuidae) (GenBank accession No. EU729322) was cloned and expressed. The target gene H.a-ALP, having an open reading frame of 1608 bp, was reverse-transcribed from cDNA by the polymerase chain reaction. The open reading frame of the target gene was cloned into the pET-32a expression vector to obtain recombinant protein in Escherichia coli DE-3 cells for the subsequent production of polyclonal antibody. New Zealand white rabbits were used for production of anti-pET-32a-H.a-ALP.

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