Electrochemical biosensor

Semi-quantitative detection of gene expression using bisbenzimide dye

P. Kittimongkolsuk, Tencomnao, T., and Santiyanont, R., Semi-quantitative detection of gene expression using bisbenzimide dye, vol. 10. pp. 3747-3759, 2011.

An electrochemical biosensor, using a disposable electrochemical printed chip aggregation by the bisbenzimide dye (Hoechst 33258), was used for detecting the expression of β-actin and RAGE genes. Using linear sweep voltammetry, the expression of these two genes in HeLa and HepG2 cell lines was determined based on anodic peak current, and the results were compared with conventional agarose gel electrophoresis. Total cellular RNA was reverse transcribed to complementary DNA, and amplification by PCR was carried out.

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