Effector protein

HydroCalc Proteome: a tool to identify distinct characteristics of effector proteins

G. J. da Silva, da Silva, R. G. T. M., Silva, V. A., Caritá, E. C., Fachin, A. L., Marins, M., da Silva, G. J., da Silva, R. G. T. M., Silva, V. A., Caritá, E. C., Fachin, A. L., and Marins, M., “HydroCalc Proteome: a tool to identify distinct characteristics of effector proteins”, vol. 15, p. -, 2016.

Bacterial pathogenicity is associated with secretion of effector proteins into intra- and extracellular spaces. These proteins interfere with cellular processes such as inhibition of phagosome-lysosome fusion, induction of apoptosis and autophagy, activation and suppression of kinases, regulation of receptor activity, and modulation of transcription factors. Knowledge regarding the characteristics of these proteins would assist in pathogenicity studies, and help to identify possible and novel targets for antibacterial drugs.

Construction of overexpression vectors of Magnaporthe oryzae genes BAS1 and BAS4 fusion to mCherry and screening of overexpression strains

M. L. Liang, Yan, J. L., Yang, Y. Q., Liu, L., Li, C. Y., and Yang, J., “Construction of overexpression vectors of Magnaporthe oryzae genes BAS1 and BAS4 fusion to mCherry and screening of overexpression strains”, vol. 14, pp. 7068-7078, 2015.

The aim of this study was to construct overexpression vectors and selecting strains of the Magnaporthe oryzae effectors BAS1 and BAS4. Primer pairs of BAS1, BAS4, and mCherry were designed based on their known nucleotide sequences. The coding sequences of BAS1 and BAS4 were amplified, and the pXY201 plasmid was selected as a template to amplify the mCherry sequence. Fragments of BAS1 and mCherry, and BAS4 and mCherry were ligated into the pCAMBIA1302 vector. The recombinant pCAMBIA-BAS1-mCherry and pCAMBIA-BAS4-mCherry plasmids were transformed into E.

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