Ecology

Proteome analysis of tobacco leaves reveals dynamic changes in protein expression among different cultivation areas

L. M. Lu, Ye, K. Y., Tang, Z. X., Liu, L., Chen, Y., Luo, J., and Huang, Y. B., Proteome analysis of tobacco leaves reveals dynamic changes in protein expression among different cultivation areas, vol. 14, pp. 15276-15284, 2015.

The leaves of tobacco plants were used to analyze differences in protein content of tobacco grown in the four main flue-cured tobacco-producing areas of Sichuan Province, China. An improved protein extraction method, isoelectric focusing/sodium dodecyl sulfate-polyacrylamide gel electrophoresis two-dimensional gel electrophoretic separation, was used to extract and separate total protein from tobacco leaves. Proteomic maps with relatively high resolution and repeatability were produced.

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