E3 ligase

Comparative fusion expression of maize SINAT5 in two different strains of Escherichia coli

A. Gholizadeh, Comparative fusion expression of maize SINAT5 in two different strains of Escherichia coli, vol. 11, pp. 2760-2768, 2012.

SINAT5 is a plant E3 ligase that regulates auxin signaling and root morphogenesis by ubiquitination of the NAC1 protein. Consequently, it may be a putative regulator of aspects of plant development cycles that are controlled by auxin. Efficient production, purification and correctly folded form of this protein are important requirements for functional studies. We produced and quantitatively compared fusion expression of the “maltose binding protein (mbp)-maize sinat5” construct in two different strains of Escherichia coli.

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