Dual Bt gene

Effect of dual Bt-expression transformation vectors on transgene expression in tobacco

L. N. Xu, Dong, Y., Zhang, J., Wang, R. X., Liu, H. M., Yang, Q., Yang, M. S., Xu, L. N., Dong, Y., Zhang, J., Wang, R. X., Liu, H. M., Yang, Q., and Yang, M. S., Effect of dual Bt-expression transformation vectors on transgene expression in tobacco, vol. 15, p. -, 2016.

This study aimed to determine the influence of vector structure on dual Bt gene expression and establish an efficient expression vector using Cry1Ac and Cry3A genes. Four vectors (N4, N5, N10, and S23) were developed and used for genetic transformation of tobacco to obtain insect-resistant transgenic lines. The vectors were constructed using the MAR structure, applying different promoter and enhancer sequences, and changing the transgene open-reading frame sequence.

Subscribe to Dual Bt gene