Dreb gene

Cloning and quantitative expression analysis of drought-induced genes in soybean

R. Stolf-Moreira, Medri, M. E., Neumaier, N., Lemos, N. G., Brogin, R. L., Marcelino, F. C., de Oliveira, M. C. N., Farias, J. R. B., Abdelnoor, R. V., and Nepomuceno, A. L., Cloning and quantitative expression analysis of drought-induced genes in soybean, vol. 9, pp. 858-867, 2010.

We determined the expression levels of DREB transcription factor (Gmdreb1) and of the genes Gmgols, Gmpip1b, Gmereb, and Gmdefensin in drought-tolerant (MG/BR46-Conquista) and drought-sensitive (BR16) genotypes of soybean, during drought. The trial was carried out in a controlled-environment chamber, set up to provide drought conditions. Sequences of Arabidopsis thaliana DREB-family proteins were used to build a phylogenetic tree through the alignment of the conserved regions near the AP2 domain.

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