Segment-specific targeting via RNA interference mediates down-regulation of OPN expression in hepatocellular carcinoma cells
“Segment-specific targeting via RNA interference mediates down-regulation of OPN expression in hepatocellular carcinoma cells”, vol. 14, pp. 14440-14447, 2015.
, Osteopontin (OPN) plays an important role in the metastasis and recurrence of tumors after resection of hepatocellular carcinoma (HCC). In this study, the down-regulation effect on OPN expression in HCC cells of RNA interference (RNAi) molecules designed to target different segments of OPN was investigated to identify a more effective site for OPN knockdown. Specific small interfering RNAs (siRNAs A, B, and C) of OPN were synthesized and transfected into an HCC cell line (HEP-G2; representing the OPNi-A, OPNi-B, and OPNi-C groups).