Dog

Identification of cDNA sequences and alternative splicing patterns of canine AMEL genes (AMELX and AMELY)

S. Q. Yan, Li, Y. M., Bai, C. Y., Guo, P. C., Si, S., Sun, J. H., and Zhao, Z. H., “Identification of cDNA sequences and alternative splicing patterns of canine AMEL genes (AMELX and AMELY)”, vol. 14, pp. 16241-16246, 2015.

Amelogenin is a major protein of the developing enamel matrix. There are two amelogenin genes (AMELX and AMELY) located on the X and Y chromosomes, respectively, in dogs. In the present study, we characterized full-length cDNAs and alternative splicing patterns of the AMEL genes in the tooth tissue of a dog by 5'- and 3'-rapid amplification of cDNA ends and AMEL-specific RT-PCR. Sequence analysis revealed that the coding regions of AMELX and AMELY were 579 and 576 bp (accession Nos. KP244310 and KP244311), respectively.

Development and evaluation of a loop-mediated isothermal amplification assay for detection of Ehrlichia canis DNA in naturally infected dogs using the p30 gene

V. C. Pinhanelli, Costa, P. N. M., Silva, G., Aguiar, D. M., Silva, C. M. L., Fachin, A. L., and Marins, M., “Development and evaluation of a loop-mediated isothermal amplification assay for detection of Ehrlichia canis DNA in naturally infected dogs using the p30 gene”, vol. 14, pp. 17885-17892, 2015.

Canine monocytic ehrlichiosis (CME) is a common tick-borne disease caused by the rickettsial bacterium Ehrlichia canis (Rickettsiales: Anaplasmataceae). In view of the different stages and variable clinical signs of CME, which can overlap with those of other infections, a conclusive diagnosis can more readily be obtained by combining clinical and hematological evaluations with molecular diagnostic methods. In this study, a loop-mediated isothermal amplification (LAMP) assay targeting the p30 gene of E. canis was developed.

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