DNA sequence quality

Evaluation of window cohabitation of DNA sequencing errors and lowest PHRED quality values

F. Prosdocimi, Peixoto, F. Cruz, and Ortega, J. Miguel, Evaluation of window cohabitation of DNA sequencing errors and lowest PHRED quality values, vol. 3, pp. 483-492, 2004.

When analyzing sequencing reads, it is important to distinguish between putative correct and wrong bases. An open question is how a PHRED quality value is capable of identifying the miscalled bases and if there is a quality cutoff that allows mapping of most errors. Considering the fact that a low quality value does not necessarily indicate a miscalled position, we decided to investigate if window-based analyses of quality values might better predict errors.

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