DNA methylation

Promoter methylation negatively correlated with mRNA expression but not tissue differential expression after heat stress

J. K. Gan, Zhang, D. X., He, D. L., Zhang, X. Q., Chen, Z. Y., and Luo, Q. B., Promoter methylation negatively correlated with mRNA expression but not tissue differential expression after heat stress, vol. 12, pp. 809-819, 2013.

DNA methylation plays a central role in gene expression. In this study, we detected the promoter methylation pattern of the chicken heat shock protein 70 (HSP70) gene and its association with messenger RNA (mRNA) expression before and after heat shock. The results showed that mRNA expression increased in response to heat stress and peaked at 3 h before dropping. Hypomethylation of the HSP70 promoter occurred in all of the groups studied, but the difference between groups within tissue type was not significant.

DNA methylation involved in proline accumulation in response to osmotic stress in rice (Oryza sativa)

C. Y. Zhang, Wang, N. N., Zhang, Y. H., Feng, Q. Z., Yang, C. W., and Liu, B., DNA methylation involved in proline accumulation in response to osmotic stress in rice (Oryza sativa), vol. 12, pp. 1269-1277, 2013.

Proline accumulation is involved in plant osmotic stress tolerance. Given that DNA methylation is related to almost all metabolic processes through regulation of gene expression, we suspected that this epigenetic modification and proline biosynthesis are probably related. To test this, we investigated whether osmotic stress-induced proline accumulation is associated with DNA methylation modifications in rice.

Search for methylation-sensitive amplification polymorphisms in mutant figs

M. G. F. Rodrigues, Martins, A. B. G., Bertoni, B. W., Figueira, A., and Giuliatti, S., Search for methylation-sensitive amplification polymorphisms in mutant figs, vol. 12, pp. 2267-2280, 2013.

Fig (Ficus carica) breeding programs that use conventional approaches to develop new cultivars are rare, owing to limited genetic variability and the difficulty in obtaining plants via gamete fusion. Cytosine methylation in plants leads to gene repression, thereby affecting transcription without changing the DNA sequence. Previous studies using random amplification of polymorphic DNA and amplified fragment length polymorphism markers revealed no polymorphisms among select fig mutants that originated from gamma-irradiated buds.

Effect of inhibition of MEK pathway on 5-aza-deoxycytidine-suppressed pancreatic cancer cell proliferation

X. Wang, Wang, H., Jiang, N., Lu, W., Zhang, X. F., and Fang, J. Y., Effect of inhibition of MEK pathway on 5-aza-deoxycytidine-suppressed pancreatic cancer cell proliferation, vol. 12, pp. 5560-5573, 2013.

Pancreatic adenocarcinoma is a lethal disease because it is inoperable at the time of diagnosis. Therefore, the search for new therapeutic approaches is critical. The abnormal expression of the mitogen-activated protein kinase/extracellular signal-regulated kinase (MEK) pathway and alteration in epigenetic modification (DNA methylation and acetylation of histones) is a common feature in the majority of human pancreatic adenocarcinomas.

Methylation status and chromatin structure of the myostatin gene promoter region in the sea perch Lateolabrax japonicus (Perciformes)

E. M. Abbas, Takayanagi, A., Shimizu, N., and Kato, M., Methylation status and chromatin structure of the myostatin gene promoter region in the sea perch Lateolabrax japonicus (Perciformes), vol. 10, pp. 3306-3315, 2011.

Myostatin is a negative regulator of the growth and development of skeletal muscle mass. In fish, myostatin is expressed in several organs in addition to skeletal muscle. To understand the mechanisms regulating myostatin gene expression in the sea perch, Lateolabrax japonicus, we examined the methylation status of the myostatin gene promoter region in several tissues (liver, eye, kidney, brain, and heart) isolated from adult specimens.

Genetic and epigenetic status of triple exotic consanguinity cotton introgression lines

S. P. He, Sun, J. L., and Du, X. M., Genetic and epigenetic status of triple exotic consanguinity cotton introgression lines, vol. 10, pp. 4063-4072, 2011.

Introgression lines are some of the most important germplasm for breeding applications and other research conducted on cotton crops. The DNA methylation level among 10 introgression lines of cotton (Gossypium hirsutum) and three exotic parental species (G. arboreum, G. thurberi and G. barbadense) were assessed by methylation-sensitive amplified polymorphism (MSAP) technology. The methylation level in the introgression lines ranged from 33.3 to 51.5%.

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