DNA markers

Molecular characterization of Anthurium genotypes by using DNA fingerprinting and SPAR markers

J. D. Souza Neto, Soares, T. C. B., Motta, L. B., Cabral, P. D. S., and Silva, J. A., Molecular characterization of Anthurium genotypes by using DNA fingerprinting and SPAR markers, vol. 13, pp. 4766-4775, 2014.

We characterized single primer amplification reaction (SPAR) molecular markers from 20 genotypes of Anthurium andraeanum Lind., including 3 from commercial varieties and 17 from 2 communities in the State of Espírito Santo, Brazil. Twenty-four SPAR, consisting of 7 random amplified polymorphic DNA and 17 inter-simple sequence repeat markers were used to estimate the genetic diversity of 20 Anthurium accessions. The set of SPAR markers generated 288 bands and showed an average polymorphism percentage of 93.39%, ranging from 71.43 to 100%.

Evaluation of genetic similarity between accessions of Pityrocarpa moniliformis (angico-de-bezerro) using RAPD markers

F. M. S. Sousa, Mendes, R. M., Mendes, R. F. M., Neto, R. B. Araújo, Nascimento, M. P. S. B. C., and Lima, P. S. C., Evaluation of genetic similarity between accessions of Pityrocarpa moniliformis (angico-de-bezerro) using RAPD markers, vol. 13, pp. 5815-5821, 2014.

Pityrocarpa moniliformis (Benth.) Luckow and Jobson, commonly known as angico-de-bezerro, is a forage legume that occurs naturally in the Caatinga of northeastern Brazil. This fast growing, vigorous, melliferous tree is well adapted to arid terrains and its branches and leaves possess high nutritional value. However, the scarcity of information regarding genetic variability within the species limits its possible exploitation as an animal forage. The aim of the study was to evaluate the genetic similarities of ten accessions of P.

Efficiency of RAPD versus SSR markers for determining genetic diversity among popcorn lines

A. A. Leal, Mangolin, C. A., Júnior, A. Tdo Amaral, Gonçalves, L. S. A., Scapim, C. A., Mott, A. S., Eloi, I. B. O., Cordovés, V., and da Silva, M. F. P., Efficiency of RAPD versus SSR markers for determining genetic diversity among popcorn lines, vol. 9, pp. 9-18, 2010.

Using only one type of marker to quantify genetic diversity generates results that have been questioned in terms of reliability, when compared to the combined use of different markers. To compare the efficiency of the use of single versus multiple markers, we quantified genetic diversity among 10 S7 inbred popcorn lines using both RAPD and SSR markers, and we evaluated how well these two types of markers discriminated the popcorn genotypes.

Estimation of genetic diversity among sunflower genotypes through random amplified polymorphic DNA analysis

M. A. Iqbal, Sadaqat, H. A., and Khan, I. A., Estimation of genetic diversity among sunflower genotypes through random amplified polymorphic DNA analysis, vol. 7, pp. 1408-1413, 2008.

The genetic diversity among eight sunflower lines was determined through the estimation of the random amplified polymorphic DNA method. One hundred and fifty-six DNA fragments were generated by 20 random primers, for an average of about 7.8 bands per primer. Of these amplified DNA fragments, 104 were polymorphic among the eight sunflower lines. Nei and Li’s similarity matrix gave values from 51.59 to 77.78%, which indicated a broad genetic base. The maximum similarity, 77.78%, was observed between R-SIN-82 and RN-46.

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