DNA marker

Viability of simultaneous genotypic screening and germination of individual seeds in rice, beans and maize

R. R. de Brito, Leão-Araújo, E. F., Ferreira, L. Bda S., L. Mendes, deM. O., Pereira, C. Cde O., da Silva, J. O., Bógea, E., Hoffmann, L. V., and de Menezes, I. P. P., “Viability of simultaneous genotypic screening and germination of individual seeds in rice, beans and maize”, Genetics and Molecular Research, vol. 18, no. 1, 2019.

Assessment of the genetic diversity and population structure of Jatropha curcas accessions in Brazil using ISSR markers

S. O. Gomes, Mendes, R. F. M., Gomes, R. L. F., Veloso, M. E. C., Arriel, N. H. C., Azevedo, D. M. P., Carvalho, G. M. C., and Lima, P. S. C., “Assessment of the genetic diversity and population structure of Jatropha curcas accessions in Brazil using ISSR markers”, Genetics and Molecular Research, vol. 17, no. 4, 2018.

An improved DNA marker technique for genetic characterization using RAMP-PCR with high-GC primers

C. L. Wei, Cheng, J. L., Khan, M. A., Yang, L. Q., Imani, S., Chen, H. C., Fu, J. J., Wei, C. L., Cheng, J. L., Khan, M. A., Yang, L. Q., Imani, S., Chen, H. C., and Fu, J. J., “An improved DNA marker technique for genetic characterization using RAMP-PCR with high-GC primers”, vol. 15, p. -, 2016.

Random amplified polymorphic DNA (RAPD) is a widely used molecular marker technique. As traditional RAPD has poor reproducibility and productivity, we previously developed an improved RAPD method (termed RAMP-PCR), which increased the reproducibility, number of bands, and efficiency of studies on polymorphism. To further develop the efficiency of this method, we used high-GC content primers for improved RAMP-PCR with DNA samples from Lonicera japonica.

Molecular characterization of twenty polymorphic microsatellite markers in the polyploid fruit tree species Syzygium samarangense (Myrtaceae)

J. M. Lai, Tsai, C. C., Yen, C. R., Ko, Y. Z., Chen, S. R., Weng, I. S., Lin, Y. S., and Chiang, Y. C., “Molecular characterization of twenty polymorphic microsatellite markers in the polyploid fruit tree species Syzygium samarangense (Myrtaceae)”, vol. 14, pp. 13013-13021, 2015.

Syzygium samarangense (Blume) Merr. & Perry (wax apple) is an important commercial fruit tree in Southeast Asia. Here, microsatellite markers were developed to evaluate genetic diversity and distinguish cultivars in this species. In total, 161 microsatellite loci with sufficient flanking sequences to design primer sets were isolated from wax apple using a magnetic bead-enrichment method. Fifty-eight primer sets were designed based on the flanking sequences of each single sequence repeat (SSR) locus and were tested using 14 wax apple cultivars/lines.

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