DNA marker

Identification of apple cultivars on the basis of simple sequence repeat markers

G. S. Liu, Zhang, Y. G., Tao, R., Fang, J. G., and Dai, H. Y., Identification of apple cultivars on the basis of simple sequence repeat markers, vol. 13, pp. 7377-7387, 2014.

DNA markers are useful tools that play an important role in plant cultivar identification. They are usually based on polymerase chain reaction (PCR) and include simple sequence repeats (SSRs), inter-simple sequence repeats, and random amplified polymorphic DNA. However, DNA markers were not used effectively in the complete identification of plant cultivars because of the lack of known DNA fingerprints. Recently, a novel approach called the cultivar identification diagram (CID) strategy was developed to facilitate the use of DNA markers for separate plant individuals.

Development of a species-specific sequence-characterized amplified region marker for roses

S. Riaz, Sadia, B., Awan, F. S., Khan, I. A., Sadaqat, H. A., and Khan, I. A., Development of a species-specific sequence-characterized amplified region marker for roses, vol. 11, pp. 440-447, 2012.

DNA fingerprints of four rose species, Rosa centifolia, R. Gruss-an-Teplitz, R. bourboniana, and R. damascena, were developed using RAPD-PCR. We identified a unique polymorphic band in R. centifolia. This 762-bp fragment was produced by the random primer GLI-2. The fragment was eluted and directly cloned in a TA cloning vector, pTZ57R/T. Digestion of the plasmid with EcoRI confirmed the cloning of GLI-2762 in pTZ57R/T.

DNA profiling of sugarcane genotypes using randomly amplified polymorphic DNA

S. Tabasum, Khan, F. A., Nawaz, S., Iqbal, M. Z., and Saeed, A., DNA profiling of sugarcane genotypes using randomly amplified polymorphic DNA, vol. 9, pp. 471-483, 2010.

DNA profiles of 40 sugarcane genotypes were constructed with 30 RAPD markers. Sugarcane genotypes of both Saccharum officinarum and S. barberi were included in this study. Multiple alleles were detected from each RAPD; there was a high level of polymorphism. On average, 7.93 alleles were produced per primer, giving a total of 238 alleles. The genetic distances between these genotypes were assessed with the POPGENE DNA sequence analysis software. A dendrogram was constructed from these data; cultivated species of sugarcane formed clusters with S.

Genetic studies of “noble cane” for identification and exploitation of genetic markers

S. Nawaz, Khan, F. A., Tabasum, S., Iqbal, M. Z., and Saeed, A., Genetic studies of “noble cane” for identification and exploitation of genetic markers, vol. 9, pp. 1011-1022, 2010.

Forty genotypes (clones) of sugarcane, including elite lines, commercial cultivars of Saccharum officinarum and clones of S. barberi were fingerprinted with 50 SSR markers using a PCR-based marker assay. Nei’s genetic distances for SSR data were determined and relationships between accessions were portrayed graphically in the form of a dendrogram. Genetic distance values ranging from 0.60 to 1.11 were observed among the 40 sugarcane accessions. The shortest genetic distance of 0.60 was seen between genotypes US-804 and US-130.

Phylogenetic relationships among Saccharum clones in Pakistan revealed by RAPD markers

S. Nawaz, Khan, F. A., Tabasum, S., Zakria, M., Saeed, A., and Iqbal, M. Z., Phylogenetic relationships among Saccharum clones in Pakistan revealed by RAPD markers, vol. 9, pp. 1673-1682, 2010.

Forty sugarcane genotypes (clones), including elite lines, commercial cultivars of Saccharum officinarum and S. barberi clones, were fingerprinted with 30 RAPD markers, using a PCR-based marker assay. The genetic distance for RAPD data was determined according to Nei, and relationships between accessions were graphed in a dendrogram. Genetic distance values ranging from 16.2 to 86.3% were observed among the 40 sugarcane accessions. The lowest genetic distance was found between genotypes US-406 and US-186.

Brazilian Nelore cattle: a melting pot unfolded by molecular genetics

M. A. C. Dani, Heinneman, M. B., and Dani, S. U., Brazilian Nelore cattle: a melting pot unfolded by molecular genetics, vol. 7, pp. 1127-1137, 2008.

The aim of the present article was to study the population structure and genetic diversity of Nelore cattle and genetic relationships between Nelore and different taurine and zebu breeds raised in Brazil. DNA polymorphism analysis was carried out with 1976 animals of 16 zebu, taurine and synthetic breeds raised in Brazil. A higher genetic differentiation was observed in taurine than in zebu cattle. Gene flow was intense between the different zebu populations.

Identification of Glomerella cingulata f. sp phaseoli recombinants by RAPD markers

O. A. Camargo Junior, Souza, E. A., Mendes-Costa, M. C., Santos, J. B., and Soares, M. A., Identification of Glomerella cingulata f. sp phaseoli recombinants by RAPD markers, vol. 6, pp. 607-615, 2007.

We examined the capacity of strains of Glomerella cin-gulata f. sp phaseoli fungus (Colletotrichum lindemuthianum sexual stage) to form recombinants, using random amplified polymorphic DNA (RAPD). Crosses of all possible combinations between strains 40, 42, 20, 21, 22, 23, 24, 25, and 26 were made on Petri dishes using M3 culture medium. The 42 x 21 cross produced the largest number of perithecia and five asci; the respective ascospores were isolated. RAPD analysis was performed on the parents and descendants.

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