DNA detection of Clostridium difficile infection based on real-time resistance measurement
“DNA detection of Clostridium difficile infection based on real-time resistance measurement”, vol. 12, pp. 3296-3304, 2013.
, We used a newly developed electrochemical method, real-time resistance measurement, based on loop-mediated isothermal amplification (LAMP), with real-time resistance monitoring and derivative analysis. DNA extracted from specimens was amplified through LAMP reaction. The 2 products of LAMP, DNA and pyrophosphate, both are negative ions; they combine with positive dye (crystal violet) and positive ions (Mg2+), which leads to an increase in the resistivity of the reaction liquid.