DNA detection

DNA detection of Clostridium difficile infection based on real-time resistance measurement

C. Liu, Jiang, D. N., Xiang, G. M., Luo, F. K., Liu, L. L., Yu, J. C., and Pu, X. Y., DNA detection of Clostridium difficile infection based on real-time resistance measurement, vol. 12, pp. 3296-3304, 2013.

We used a newly developed electrochemical method, real-time resistance measurement, based on loop-mediated isothermal amplification (LAMP), with real-time resistance monitoring and derivative analysis. DNA extracted from specimens was amplified through LAMP reaction. The 2 products of LAMP, DNA and pyrophosphate, both are negative ions; they combine with positive dye (crystal violet) and positive ions (Mg2+), which leads to an increase in the resistivity of the reaction liquid.

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