DNA amplification

Molecular characterization and RAPD analysis of Juniperus species from Iran

J. Kasaian, Behravan, J., Hassany, M., Emami, S. A., Shahriari, F., and Khayyat, M. H., “Molecular characterization and RAPD analysis of Juniperus species from Iran”, vol. 10, pp. 1069-1074, 2011.

The genus Juniperus L. (Cupressaceae), an aromatic evergreen plant, consists of up to 68 species around the world. We classified five species of Juniperus found in Iran using molecular markers to provide a means for molecular identification of Iranian species. Plants were collected (three samples of each species) from two different provinces of Iran (Golestan and East Azarbayejan). The DNA was extracted from the leaves using a Qiagen Dneasy Plant Mini Kit. Amplification was performed using 18 ten-mer RAPD primers.

Single primer-based DNA amplification as a suitable and low-cost tool for assessing genetic diversity in mangrove crabs

F. B. Britto, Mendes, D. S. F., Ogawa, M., Cintra, I. H. A., and Diniz, F. M., “Single primer-based DNA amplification as a suitable and low-cost tool for assessing genetic diversity in mangrove crabs”, vol. 10. pp. 4084-4092, 2011.

We used single primer-based DNA markers to assess genetic variability of the mangrove crab, Ucides cordatus, collected from four different localities from Pará to Santa Catarina States in Brazil (almost 5000 km distant). Five primers were chosen based on the consistency of the amplified bands and the polymorphism of each locus. A total of 78 loci were amplified in 76 samples; high polymorphism rates were detected in the entire sample (80.8%) and within each locality (73.5-79.5%).

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