Development-related genes

H3K27me3 may be associated with Oct4 and Sox2 in mouse preimplantation embryos

F. - R. Wu, Zhang, Y., Ding, B., Lei, X. - H., Huang, J. - C., Wang, C. - H., Liu, Y., Wang, R., and Li, W. - Y., H3K27me3 may be associated with Oct4 and Sox2 in mouse preimplantation embryos, vol. 13, pp. 10121-10129, 2014.

As a core member of polycomb repressive complex 2, the transcription and enzyme activity of enhancer of zeste homolog 2 (Ezh2) is directly involved in the trimethylation of lysine 27 on histone H3. In this study, the fluorescence intensity of H3K27me3 in mouse in vivo morulae and blastocysts was compared by indirect immunofluorescence staining. We found that demethylation of H3K27me3 occurred during the blastocyst stage. Real-time polymerase chain reaction was performed to investigate Ezh2 expression in oocytes and in preimplantation embryos.

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