Dengue

Drosha, DGCR8, and Dicer mRNAs are down-regulated in human cells infected with dengue virus 4, and play a role in viral pathogenesis

S. M. M. Casseb, Simith, D. B., Melo, K. F. L., Mendonça, M. H., Santos, A. C. M., Carvalho, V. L., Cruz, A. C. R., Vasconcelos, P. F. C., Casseb, S. M. M., Simith, D. B., Melo, K. F. L., Mendonça, M. H., Santos, A. C. M., Carvalho, V. L., Cruz, A. C. R., and Vasconcelos, P. F. C., Drosha, DGCR8, and Dicer mRNAs are down-regulated in human cells infected with dengue virus 4, and play a role in viral pathogenesis, vol. 15, p. -, 2016.

Dengue virus (DENV) and its four serotypes (DENV1-4) belong to the Flavivirus genus of the Flaviviridae family. DENV infection is a life-threatening disease, which results in up to 20,000 deaths each year. Viruses have been shown to encode trans-regulatory small RNAs, or microRNAs (miRNAs), which bind to messenger RNA and negatively regulate host or viral gene expression. During DENV infections, miRNAs interact with proteins in the RNAi pathway, and are processed by ribonucleases such as Dicer and Drosha.

Optimization of dengue virus genome assembling using GSFLX 454 pyrosequencing data: evaluation of assembling strategies

S. M. M. Casseb, Cardoso, J. F., Ramos, R., Carneiro, A., Nunes, M., Vasconcelos, P. F. C., and Silva, A., Optimization of dengue virus genome assembling using GSFLX 454 pyrosequencing data: evaluation of assembling strategies, vol. 11, pp. 3688-3695, 2012.

Currently assembling genomes without reference is one of the most important challenges for bioinformaticists all over the world in an attempt to characterize new organisms. The current study has used two dengue virus type 4 (DENV-4) strains recently isolated in Brazil, which have its genomes sequenced using the GSFLX 454 sequencer (Roche, Life Science) by the pyrosequencing method. The GSFLX 454 data were used for testing different genome assembling strategies.

Potential control of Aedes aegypti (Diptera: Culicidae) with Piper aduncum L. (Piperaceae) extracts demonstrated by chromosomal biomarkers and toxic effects on interphase nuclei

M. S. Rafael, Hereira-Rojas, W. J., Roper, J. J., Nunomura, S. M., and Tadei, W. P., Potential control of Aedes aegypti (Diptera: Culicidae) with Piper aduncum L. (Piperaceae) extracts demonstrated by chromosomal biomarkers and toxic effects on interphase nuclei, vol. 7, pp. 772-781, 2008.

Dillapiol, a phenylpropanoid isolate from essential oils of leaves of Piper aduncum (Piperaceae), has insecticidal, fungicidal and antimicrobial activities. The insecticidal activity of dil lapiol was tested in vivo on the larvae and pupae of Aedes aegypti, the mosquito vector of dengue. Specifically, the effect of dillapiol on the formation of micronuclei and chromosome aberrations was analyzed.

Reduced levels of genetic variation in Aedes albopictus (Diptera: Culicidae) from Manaus, Amazonas State, Brazil, based on analysis of the mitochondrial DNA ND5 gene

R. T. Maia, Scarpassa, V. M., Maciel-Litaiff, L. H., and Tadei, W. P., Reduced levels of genetic variation in Aedes albopictus (Diptera: Culicidae) from Manaus, Amazonas State, Brazil, based on analysis of the mitochondrial DNA ND5 gene, vol. 8, pp. 998-1007, 2009.

Aedes albopictus, a mosquito originally from Southeast Asia, is considered to be one of the main vectors of dengue fever, yel­low fever and other arboviruses. We examined the genetic variability and population structure of 68 individuals of Ae. albopictus collected from five neighborhoods of the city of Manaus, based on the mitochon­drial gene coding for NADH dehydrogenase subunit 5 (ND5).

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