Degenerate primers

Molecular cloning and characterization of the full-length Hsp90 gene from Matricaria recutita

S. P. Ling, Su, S. S., Zhang, H. M., Zhang, X. S., Liu, X. Y., Pan, G. F., and Yuan, Y., Molecular cloning and characterization of the full-length Hsp90 gene from Matricaria recutita, vol. 13, pp. 10994-11003, 2014.

Heat shock protein 90 (Hsp90) is one of the most abundant and conserved chaperone proteins and plays important roles in plant growth and responses to environmental stimuli. However, little is known regarding the sequence and function of Hsp90s in Matricaria recutita. In the present study, we cloned the full-length cDNA sequence of the hsp90 gene from this species.

Development of universal primers for isolating fragments of the LEAFY gene

L. X. Yu and Yan, B., Development of universal primers for isolating fragments of the LEAFY gene, vol. 12. pp. 1777-1780, 2013.

LEAFY orthologs are found throughout land plants. Degenerate primers were designed for amplifying exon 3 region fragments of the LEAFY gene. Sixteen taxa from different plants were sampled, all of which were successfully isolated, which indicated that this set of primers is universally applicable for all land plants.

Simple and efficient method for isolating cDNA fragments of lea3 genes with potential for wide application in the grasses (Poaceae)

L. Yu, Wu, X., Tang, X., and Yan, B., Simple and efficient method for isolating cDNA fragments of lea3 genes with potential for wide application in the grasses (Poaceae), vol. 9. pp. 1321-1325, 2010.

cDNA fragments of lea3 genes with a high GC content (from 68 to 77%) were found in several Poaceae, including Sorghum vulgare, Saccharum officinarum, Oryza officinalis, Oryza meyeriana, Ampelocalamus calcareus, Cynodon dactylon, and Zizania latifoli. They were successfully isolated by means of optimal experimental parameters, which included dimethyl sulfoxide as additive and degenerate primers “AGETKAS” and “AGKDKTG”, and their sequences were analyzed.

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