De novo transcriptome

Transcriptome analysis of potential simple sequence repeat markers in Ammopiptanthus mongolicus

M. Jin, Guo, M. Y., Han, L., Li, J. L., Yang, S. Y., Su, Y. H., Jin, M., Guo, M. Y., Han, L., Li, J. L., Yang, S. Y., and Su, Y. H., Transcriptome analysis of potential simple sequence repeat markers in Ammopiptanthus mongolicus, vol. 15, p. -, 2016.

Ammopiptanthus mongolicus, an evergreen broadleaf legume shrub, can survive under conditions of high and low temperature, extreme salinity, and drought. This attribute makes it an ideal model for studying mechanisms of stress tolerance in plants. However, simple sequence repeat (SSR) resources for this species are insufficient in public databases. In this study, a total of 44,959 unigenes identified from the A. mongolicus transcriptome were used for SSR analysis by MIcroSAtellite (MISA).

Screening potential SSR markers of the anadromous fish Coilia nasus by de novo transcriptome analysis using Illumina sequencing

D. - A. Fang, Zhou, Y. - F., Duan, J. - R., Zhang, M. - Y., Xu, D. - P., Liu, K., Xu, P., and Wei, Q., Screening potential SSR markers of the anadromous fish Coilia nasus by de novo transcriptome analysis using Illumina sequencing, vol. 14, pp. 14181-14188, 2015.

RNA-Seq technology has been widely applied to tran­scriptomics, genomics, molecular marker development, and functional gene studies. In the genome, microsatellites are simple sequence re­peats (SSR) with a high degree of polymorphism that are used as DNA markers in many molecular genetic studies. Using traditional methods such as magnetic bead enrichment, only a few microsatellite markers have been isolated. Coilia nasus is an anadromous, small-to-moder­ately sized fish species that is famous as an important fishery resource.

Subscribe to De novo transcriptome