Culture Technology

DKK1 eukaryotic expression plasmid and expression product identification

G. Y. Bao, Lu, K. Y., Cui, S. F., and Xu, L., DKK1 eukaryotic expression plasmid and expression product identification, vol. 14, pp. 6312-6318, 2015.

We constructed the human dickkopf 1 (DKK1) eukaryotic expression plasmid and expressed, purified, and identified its expression product. We extracted cancer cells from cervical cancer tissue, followed by extraction of mRNA. Reverse transcription-polymerase chain reaction was conducted to obtain DKK1 gene fragments. Using these fragments, we prepared the recombinant plasmid pCMV-HA2/DKK1. The recombinant plasmid was restriction enzyme-digested and sequenced, and using liposome vectors, was transiently transfected into Free-Style 293-F cells (serum-free medium).

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