Culture conditions

Differences in H3K4 trimethylation in in vivo and in vitro fertilization mouse preimplantation embryos

F. - R. Wu, Liu, Y., Shang, M. - B., Yang, X. - X., Ding, B., Gao, J. - G., Wang, R., and Li, W. - Y., Differences in H3K4 trimethylation in in vivo and in vitro fertilization mouse preimplantation embryos, vol. 11, pp. 1099-1108, 2012.

Trimethylation of lysine 4 at histone 3 (H3K4me3) is considered a marker of active transcription; it plays an important role in transcription reprogramming efficiency. We compared the levels of H3K4me3 in mouse preimplantation embryos from MII stage oocytes produced by in vivo and in vitro fertilization (IVF) using immunofluorescence histochemistry. IVF embryos were further treated with trichostatin A (a histione deacetylase inhibitor) to investigate the effect of histone acetylation on H3K4me3.

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