Cultivar identification

A novel random amplified polymorphic DNA-based strategy for genetic diversity analysis and identification of tomatoes

X. Cao, Wu, Z., Zhou, R., Jiang, F. L., and Yang, Z. E., A novel random amplified polymorphic DNA-based strategy for genetic diversity analysis and identification of tomatoes, vol. 14, pp. 1650-1661, 2015.

Cultivar identification diagrams (CIDs) provide a rapid and efficient approach for identifying cultivars based on random amplified polymorphic DNA (RAPD) markers. In this paper, 64 tomato cultivars were identified using a CID. Using RAPD profiles, clustering analysis was performed to analyze genetic diversity. The results showed that 8 RAPD primers could completely separate the 64 cultivars according to the obtained polymorphic bands; a CID of the 64 tomato cultivars was then constructed.

A new strategy for complete identification of sea buckthorn cultivars by using random amplified polymorphic DNA markers

G. Yang, Ding, J., Wu, L. R., Duan, Y. D., Li, A. Y., Shan, J. Y., and Wu, Y. X., A new strategy for complete identification of sea buckthorn cultivars by using random amplified polymorphic DNA markers, vol. 14, pp. 1836-1845, 2015.

DNA fingerprinting is both a popular and important technique with several advantages in plant cultivar identification. However, this technique has not been used widely and efficiently in practical plant identification because the analysis and recording of data generated from fingerprinting and genotyping are tedious and difficult. We developed a novel approach known as a cultivar identification diagram (CID) strategy that uses DNA markers to separate plant individuals in a more efficient, practical, and referable manner.

Genetic relationships analysis of olive cultivars grown in China

M. M. Zhan, Cheng, Z. Z., Su, G. C., Wang, A. Y., Chen, H. P., Yang, Z. S., Shan, Z., and Huang, Q. M., Genetic relationships analysis of olive cultivars grown in China, vol. 14, pp. 5958-5969, 2015.

The olive tree is an iconic tree of the Mediterranean, and is used extensively to produce high-quality olive oil. Although the China olive industry has just begun to be valued, there were also existed mislabeling and synonyms in introduced cultivars. The aim of this study was to analyze genetic similarities among olive cultivars in China using SSR and ISSR techniques. Thirty-two samples were collected from Xichang. Five of these cultivars were issued from a Chinese breeding program.

Efficient identification of ornamental peach cultivars using RAPD markers with a manual cultivar identification diagram strategy

J. Han, Wang, W. Y., Leng, X. P., Guo, L., Yu, M. L., Jiang, W. B., and Ma, R. J., Efficient identification of ornamental peach cultivars using RAPD markers with a manual cultivar identification diagram strategy, vol. 13, pp. 32-42, 2014.

One of the most important uses of DNA markers is cultivar identification. However, no DNA fingerprint analysis strategy is available for making DNA markers helpful in practical plant cultivar identification, especially for the identification of a large number of cultivars. We developed a manual cultivar identification diagram strategy for efficient identification of plant cultivars, from which a cultivar identification diagram (CID) of genotyped plant individuals can be constructed manually.

Analysis of genetic relationships and identification of lily cultivars based on inter-simple sequence repeat markers

G. F. Cui, Wu, L. F., Wang, X. N., Jia, W. J., Duan, Q., Ma, L. L., Jiang, Y. L., and Wang, J. H., Analysis of genetic relationships and identification of lily cultivars based on inter-simple sequence repeat markers, vol. 13, pp. 5778-5786, 2014.

Inter-simple sequence repeat (ISSR) markers were used to discriminate 62 lily cultivars of 5 hybrid series. Eight ISSR primers generated 104 bands in total, which all showed 100% polymorphism, and an average of 13 bands were amplified by each primer. Two software packages, POPGENE 1.32 and NTSYSpc 2.1, were used to analyze the data matrix.

An improved strategy based on RAPD markers efficiently identified 95 peach cultivars

M. L. Yu, Wang, W. Y., Ma, R. J., Shen, Z. J., and Fang, J. G., An improved strategy based on RAPD markers efficiently identified 95 peach cultivars, vol. 11. pp. 1158-1168, 2012.

DNA markers have useful applications in cultivar identification. A novel analysis approach called cultivar identification diagram (CID) was developed using DNA markers in the separation of plant individuals. This new strategy is less time- and cost-consuming, has reliable results, and was constructed for fingerprinting. Ten 11-mer primers were used to amplify the genotypes; all 95 peach genotypes (from the National Peach Germplasm Repository, in Nanjing, China) were distinguished by a combination of 54 primers.

A new strategy employed for identification of sweet orange cultivars with RAPD markers

X. Sun, Mu, Q., Jiang, D., Wang, C., Wang, X. C., and Fang, J. G., A new strategy employed for identification of sweet orange cultivars with RAPD markers, vol. 11. pp. 2071-2080, 2012.

We optimized RAPD techniques by increasing the length of RAPD primers and performing a strict screening of PCR annealing temperature to distinguish 60 sweet orange cultivars from the Research Institute of Pomology at the Chinese Academy of Agricultural Sciences. A new approach called cultivar identification diagram (CID) was used to improve the efficiency of RAPD markers for cultivar identification.

Identification of earl millet cultivars using both microsatellites and enzymatic markers

R. P. Mendonça Neto, Von Pinho, E. V. R., Carvalho, B. L., and Pereira, G. S., Identification of earl millet cultivars using both microsatellites and enzymatic markers, vol. 12, pp. 1-14, 2013.

The increasing number of protected and registered cultivars and problems involving seed commercialization make distinction and identification of cultivars imperative. Millet (Pennisetum glaucum), a crop species with protected cultivars in Brazil, has been the target of seed piracy.

An efficient approach to identify Ginkgo biloba cultivars by using random amplified polymorphic DNA markers with a manual cultivar identification diagram strategy

G. P. Li, Zhang, C. Q., and Cao, F. L., An efficient approach to identify Ginkgo biloba cultivars by using random amplified polymorphic DNA markers with a manual cultivar identification diagram strategy, vol. 12. pp. 175-182, 2013.

Cultivar identification is a key step to avoid the formation of homonyms and synonyms of Ginkgo biloba. In this study, a new approach based on combinational utilization of polymorphic bands produced from 6 different random amplified polymorphic DNA (RAPD) primers was developed for identifying 42 Ginkgo cultivars, and a manual cultivar identification diagram that consisted of polymorphic bands produced from different RAPD primers was reported.

Development of a novel and efficient strategy for practical identification of Pyrus spp (Rosaceae) cultivars using RAPD fingerprints

J. Lin, Wang, X. C., Chang, Y. H., and Fang, J. G., Development of a novel and efficient strategy for practical identification of Pyrus spp (Rosaceae) cultivars using RAPD fingerprints, vol. 10, pp. 932-942, 2011.

Accurate and reliable cultivar identification of crop species is essential to guarantee plant material identity for purposes of registration, cultivar protection and production. To facilitate identification of plant cultivars, we developed a novel strategy for efficient recording of DNA molecular fingerprints in genotyped plant individuals. These fingerprints can be used as efficient referential information for quick plant identification. We made a random amplified polymorphic DNA (RAPD) marker analysis of 68 pear cultivars.

Subscribe to Cultivar identification