Cross-species amplification

Development of polymorphic microsatellite loci in Odontobutis obscura using Illumina paired-end sequencing and the test of cross-species amplification

H. - G. Bae, An, J., Jun, J., Kweon, S. - M., Kim, S., Yu, J. - N., Kim, B. - J., Sakai, H., Lee, Y. J., Suk, H. Y., Bae, H. - G., An, J., Jun, J., Kweon, S. - M., Kim, S., Yu, J. - N., Kim, B. - J., Sakai, H., Lee, Y. J., and Suk, H. Y., Development of polymorphic microsatellite loci in Odontobutis obscura using Illumina paired-end sequencing and the test of cross-species amplification, vol. 15, p. -, 2016.

Odontobutis obscura is a bottom-dwelling freshwater fish native to East Asia. Its range encompasses southwest China, western Japan, and Geoje Island in South Korea. Despite its widespread range in China and Japan, only a small and spatially isolated population is found in South Korea. We developed a total of 23 novel and polymorphic microsatellite loci of O. obscura using Illumina paired-end shotgun sequencing and characterized them using 80 Japanese and Korean samples.

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