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Construction of a natural phage antibody library of human anaplastic thyroid carcinoma

J. M. Xi, Pang, H., Hu, X. L., and Wang, Z. J., Construction of a natural phage antibody library of human anaplastic thyroid carcinoma, vol. 14, pp. 8397-8406, 2015.

The objective of this study was to identify and construct a human natural phage single-chain antibody (scFv) library of human anaplastic thyroid carcinoma (ATC) using phage display technology. Total RNA was extracted from lymphatic tissue near an ATC and used to amplify variable heavy chain (VH) and variable light chain (VL) fragments with added linker sequences using reverse transcription-polymerase chain reaction (RT-PCR).

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