Conservation

Genetic diversity analysis of Croton antisyphiliticus Mart. using AFLP molecular markers

T. G. Oliveira, Pereira, A. M. S., Coppede, J. S., França, S. C., Ming, L. C., Bertoni, B. W., Oliveira, T. G., Pereira, A. M. S., Coppede, J. S., França, S. C., Ming, L. C., and Bertoni, B. W., Genetic diversity analysis of Croton antisyphiliticus Mart. using AFLP molecular markers, vol. 15, p. -, 2016.

Croton antisyphiliticus Mart. is a medicinal plant native to Cerrado vegetation in Brazil, and it is popularly used to treat urogenital tract infections. The objective of the present study was to assess the genetic variability of natural C. antisyphiliticus populations using AFLP molecular markers. Accessions were collected in the states of Minas Gerais, São Paulo, and Goiás. The genotyping of individuals was performed using a LI-COR® DNA Analyzer 4300. The variability within populations was found to be greater than the variability between them.

Natural hybridization between Gossypium mustelinum and exotic allotetraploid cotton species

I. P. P. de Menezes, da Silva, J. O., Malafaia, G., Silveira, R. D. D., and Barroso, P. A. V., Natural hybridization between Gossypium mustelinum and exotic allotetraploid cotton species, vol. 14. pp. 14177-14180, 2015.

Cotton has been collected in Brazil for decades for its conservation, evaluation, and the use of its genetic resources. Gossypium mustelinum is an allotetraploid cotton species that only occurs in Brazil, and little is known about its genetic potential for improvement. However, the species is threatened by habitat fragmentation and interspecific hybridization with exotic species of cotton. In this study, we investigated the rate of natural hybridization in two populations of G. mustelinum in Bahia, Brazil, with G. hirsutum and G.

Identification and characterization of polymorphic microsatellite loci in the red-crowned crane

L. Zhang, Zhang, Z. H., Shen, F. J., Hou, R., Zhang, W. P., Liu, Y. L., Tu, K. Y., and Yang, A. L., Identification and characterization of polymorphic microsatellite loci in the red-crowned crane, vol. 14, pp. 15169-15176, 2015.

We isolated and characterized microsatellite loci for the red-crowned crane (Grus japonensis) from a microsatellite-enriched database, which was obtained using high-throughput sequencing technology. We designed primer sets for 445 microsatellite loci and after initial screening, 34 loci were genotyped in 31 red-crowned cranes. The number of observed alleles ranged from 3 to 10. Observed and expected heterozygosities ranged from 0.197 to 0.935 and 0.453 to 0.887, respectively; the mean polymorphic information content was 0.663.

Efficient development of polymorphic microsatellite loci for Pteroceltis tatarinowii (Ulmaceae)

X. H. Li, Zhang, X. P., Liu, K., Liu, H. J., and Shao, J. W., Efficient development of polymorphic microsatellite loci for Pteroceltis tatarinowii (Ulmaceae), vol. 14, pp. 16444-16449, 2015.

Pteroceltis tatarinowii (Ulmaceae) is a scientifically and economically important temperate deciduous tree that is endemic to China. In the present study, 12 P. tatarinowii polymorphic microsatellite loci were developed using the tailed primer-M13-simple sequence repeats (TP-M13-SSR) biotin-capture method. The number of alleles per locus ranged from 2 to 10, with an average of 6.58. The observed and expected heterozygosity ranged from 0.208 to 0.958 and from 0.198 to 0.858, with average values of 0.703 and 0.710, respectively.

Genetic diversity and structure in natural populations of Maytenus truncata Reiss, 1861, a medicinal plant vulnerable to extractivism in Bahia State, Brazil

R. R. Simplicio, Waldschmidt, A. M., Amorim, M. B., Almeida, B. S., and Pereira, D. G., Genetic diversity and structure in natural populations of Maytenus truncata Reiss, 1861, a medicinal plant vulnerable to extractivism in Bahia State, Brazil, vol. 14, pp. 18241-18248, 2015.

Maytenus truncata (Celastraceae) is a plant species widely used in the treatment of ulcers and tumors. Despite the intensive harvest of native specimens in the State of Bahia, northeastern Brazil, there is no information about the genetic variability or structure of this species. Therefore, the goal of this study was to estimate the genetic diversity and population structure of M. truncata based on inter simple sequence repeat (ISSR) molecular markers.

Genetic diversity of wild Prunus cerasifera Ehrhart (wild cherry plum) in China revealed by simple-sequence repeat markers

Y. Zhao, Li, Y., Liu, Y., and Yang, Y. F., Genetic diversity of wild Prunus cerasifera Ehrhart (wild cherry plum) in China revealed by simple-sequence repeat markers, vol. 14, pp. 8407-8413, 2015.

Simple-sequence repeat (SSR) markers were employed to assess the genetic diversity of wild Prunus cerasifera Ehrhart (wild cherry plum) in China. Fourteen SSR primer pairs generated a total of 94 alleles (90 were polymorphic, accounting for 95.74%), with a mean of 6.71 alleles per locus. The number of alleles detected at each locus ranged from 2 at BPPCT 028 to 13 at BPPCT 002, with an average of 6.71 alleles per locus.

Use of microsatellite markers to assign goats to their breeds

R. S. Aljumaah, Alobre, M. M., and Al-Atiyat, R. M., Use of microsatellite markers to assign goats to their breeds, vol. 14, pp. 9071-9080, 2015.

We investigated the potential of 17 microsatellite markers for assigning Saudi goat individuals to their breeds. Three local breeds, Bishi, Jabali, and Tohami were genotyped using these markers, and Somali goats were used as a reference breed. The majority of alleles were shared between the breeds, except for some that were specific to each breed. The Garza-Williamson index was lowest in the Bishi breed, indicating that a recent bottleneck event occurred.

Isolation and characterization of microsatellite loci in Branchiostoma belcheri Gray (Amphioxus)

Z. B. Li, Huang, Y. S., Shangguan, J. B., Ning, Y. F., Yi, Y., and Dai, G., Isolation and characterization of microsatellite loci in Branchiostoma belcheri Gray (Amphioxus), vol. 14, pp. 10224-10227, 2015.

Branchiostoma belcheri Gray is a second-class, nationally protected protochordate in China. We developed 10 novel polymorphic sites in B. belcheri, which were examined using a population of 30 wild individuals from Xiamen, China. The polymorphism information content ranged between 0.141 and 0.681, and the number of alleles varied from 2 to 5. The expected and observed heterozygosities varied between 0.1528 and 0.6920, and between 0.1429 and 0.5000, respectively. These novel microsatellite markers will facilitate the genetic analysis and protection of wild B.

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