Comet assay

Detection of protein and DNA damage induced by elevated carbon dioxide and ozone in Triticum aestivum L. using biomarker and comet assay

E. Abdelhaliem, Al-Huqail, A. A., Abdelhaliem, E., and Al-Huqail, A. A., Detection of protein and DNA damage induced by elevated carbon dioxide and ozone in Triticum aestivum L. using biomarker and comet assay, vol. 15, p. -, 2016.

This study was designed to compare the genetic effects of elevated carbon dioxide (CO2) and ozone (O3), alone or in combination, under irrigated and non-irrigated conditions on proteins and DNA of wheat (Triticum aestivum L.). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), isozymes, random amplified polymorphic DNA (RAPD), and comet assays were used.

Hyperproliferative action of bovine papillomavirus: genetic and histopathological aspects

R. P. Araldi, Melo, T. C., Neves, A. C., Spadacci-Morena, D. D., Magnelli, R. F., Modolo, D. G., de-Sá-Júnior, P. L., Mazucchelli-de-Souza, J., Carvalho, R. F., Beçak, W., and Stocco, R. C., Hyperproliferative action of bovine papillomavirus: genetic and histopathological aspects, vol. 14, pp. 12942-12954, 2015.

The bovine papillomavirus (BPV) causes papillomas that regress spontaneously, but can also progress to malignancy. This study evaluated the role of BPV in oncogenesis. Twenty-four samples from uninfected calves and the papillomas of BPV infected cattle were subjected to molecular diagnosis, as well as histopathological and immunohistochemical analyses. The comet assay (CA) was used to evaluate the clastogenic potential of BPV. The results confirmed the presence of BPV-2, 3, 5, and 9 in infected samples.

Castor bean (Ricinus communis L.) as a potential environmental bioindicator

M. G. Mendes, Junior, C. D. Santos, Dias, A. C. C., and Bonetti, A. M., Castor bean (Ricinus communis L.) as a potential environmental bioindicator, vol. 14, pp. 12880-12887, 2015.

Biomonitoring of air quality using living organisms is a very interesting approach to environmental impact assessment. Organisms with a vast distribution, such as plants, are widely used for these purposes. The castor bean (Ricinus communis L.) is an oleaginous plant that can potentially be used as a bioindicator plant owing to its rapid growth and large leaves, which have a wide surface area of contact with the air and the pollutants therein. This study investigated the the bioindicator potential of the castor bean by performing several tests.

Evaluation of the genotoxic activity of dicamba and atrazine herbicides in several Mexican and South American varieties of sweetcorn (Zea mays L.)

M. S. Reynoso, Alvarez, C. M., De la Cruz, L., Escoto, M. D., and Sánchez, J. J. G., Evaluation of the genotoxic activity of dicamba and atrazine herbicides in several Mexican and South American varieties of sweetcorn (Zea mays L.), vol. 14, pp. 16585-16593, 2015.

Corn is a major crop and various herbicides are used to maximize its production, which include a dicamba-atrazine mixture. This has great advantages, but can also induce DNA damage. Genotoxic activity was assessed by comet assay following application of two concentrations of dicamba-atrazine: 1000-2000 and 2000-4000 ppm. Apical meristem leaf nuclei from 119 varieties of sweetcorn plants from Mexico and South America, and from five commercial sweetcorn hybrids were used. Each accession comprised two individuals per concentration and two controls.

Bos taurus papillomavirus activity in peripheral blood mononuclear cells: demonstrating a productive infection

T. C. Melo, Araldi, R. P., Pessoa, N. S. D., de-Sá-Júnior, P. L., Carvalho, R. F., Beçak, W., and Stocco, R. C., Bos taurus papillomavirus activity in peripheral blood mononuclear cells: demonstrating a productive infection, vol. 14, pp. 16712-16727, 2015.

Bovine papillomavirus (BPV) is an oncogenic virus with mucous and epithelial tropism. Possible productive virus infection in other tissues, such as blood, has been hypothesized. In order to investigate this possibility, three samples of skin papillomas and blood were collected from bovines with BPV infection and five samples of peripheral blood and one sample of normal tissue were collected from a calf without BPV infection.

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