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Cloning and characterization of 5-enopyruvylshikimate-3-phosphate synthase from Pantoea sp

F. Liu and Cao, Y. P., Cloning and characterization of 5-enopyruvylshikimate-3-phosphate synthase from Pantoea sp, vol. 14, pp. 19233-19241, 2015.

The shikimate pathway enzyme 5-enopyruvylshikimate-3-phosphate synthase (EPSPS) is the target of the broad spectrum herbicide glyphosate. A novel aroA gene encoding an EPSPS from Pantoea sp was identified and subcloned into the pET-28a vector to construct the recombinant pET-AroAPantoea sp plasmid. Amino acid sequence analysis indicated that AroAPantoea sp is a class I AroA enzyme. When expressed in Escherichia coli, it conveyed high tolerance to glyphosate.

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