CHSP

Functional characterization of the Ginkgo biloba chalcone synthase gene promoter in transgenic tobacco

L. L. Li, Cheng, H., Yuan, H. H., Xu, F., Cheng, S. Y., and Cao, F. L., Functional characterization of the Ginkgo biloba chalcone synthase gene promoter in transgenic tobacco, vol. 13, pp. 3446-3460, 2014.

The regulative sequence (2273 bp) of the chalcone synthase gene promoter of biloba was cloned by genomic walking. A 2273-bp promoter 5' upstream translation start site of GbCHS was cloned and designated as GbCHSP. pBI121+CHSP:GUS and pBI121-35S:GUS were constructed and transformed into tobacco by LBA4404. We found that GbCHSP could drive transient expression of GUS in tobacco and differentially expressed in root, stem and leaf tissues of this plant.

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