Chrysanthemum

Isolation and characterization of the Chrysanthemum nitrate transporter CmNRT1

C. S. Gu, Zhang, X. X., Chen, S. M., Li, T., Chen, Y., Jiang, J. F., Chen, F. D., Gu, C. S., Zhang, X. X., Chen, S. M., Li, T., Chen, Y., Jiang, J. F., Chen, F. D., Gu, C. S., Zhang, X. X., Chen, S. M., Li, T., Chen, Y., Jiang, J. F., and Chen, F. D., Isolation and characterization of the Chrysanthemum nitrate transporter CmNRT1, vol. 15, p. -, 2016.

In this study, the nitrate transporter gene CmNRT1 was isolated from the chrysanthemum variety ‘Nannongxuefeng’. The full-length cDNA contains an open reading frame of 1761 bp encoding 587 residues. Using qRT-PCR, we found that CmNRT1 was induced by 10 mM NO3- in roots and shoots. Two Arabidopsis thaliana transgenic plants expressing CmNRT1 were selected for functional analyses. Root 15N influx in wild-type and transgenic A. thaliana lines under 10 or 0.2 mM 15NO3 was tested.

Comparative analysis of genetic diversity among species of Chrysanthemum and its related genera using inter-simple sequence repeat and sequence-related amplified polymorphism markers

X. Zhang, Zhang, F., Zhao, H., Guan, Z., Chen, S., Jiang, J., Fang, W., and Chen, F., Comparative analysis of genetic diversity among species of Chrysanthemum and its related genera using inter-simple sequence repeat and sequence-related amplified polymorphism markers, vol. 13, pp. 8469-8479, 2014.

In this study, inter-simple sequence repeats (ISSRs) and sequence-related amplified polymorphism (SRAP) were applied to assess the genetic diversity in 38 species of Chrysanthemum and related genera. A total of 204 and 567 bands were amplified by 24 ISSR and 25 SRAP primers, of which 196 (97%) and 557 (99%) were polymorphic, respectively. The ISSR-based genetic similarity ranged from 0.016 to 0.886 and averaged 0.201, while the SRAP-based genetic similarity varied from 0.010 to 0.811 and averaged 0.122.

Phylogenetic relationships of chrysanthemums in Korea based on novel SSR markers

A. A. Khaing, Moe, K. T., Hong, W. J., Park, C. S., Yeon, K. H., Park, H. S., Kim, D. C., Choi, B. J., Jung, J. Y., Chae, S. C., Lee, K. M., and Park, Y. J., Phylogenetic relationships of chrysanthemums in Korea based on novel SSR markers, vol. 12, pp. 5335-5347, 2013.

Chrysanthemums are well known for their esthetic and medicinal values. Characterization of chrysanthemums is vital for their conservation and management as well as for understanding their genetic relationships. We found 12 simple sequence repeat markers (SSRs) of 100 designed primers to be polymorphic. These novel SSR markers were used to evaluate 95 accessions of chrysanthemums (3 indigenous and 92 cultivated accessions). Two hundred alleles were identified, with an average of 16.7 alleles per locus.

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