Chloroplast simple sequence repeat

Mating system patterns of natural populations of Pinus koraiensis along its post-glacial colonization route in northeastern China

X. Sui, Feng, F. J., Zhao, D., Xing, M., Sun, X. Y., Han, S. J., and Li, M. H., Mating system patterns of natural populations of Pinus koraiensis along its post-glacial colonization route in northeastern China, vol. 14, pp. 4113-4124, 2015.

To understand the genetic mechanisms underlying the endangerment of Pinus koraiensis, we studied the mating system of 49 families of this species in 3 natural populations along its post-glacial colonization route across ~1500 km in northeastern China using the chloroplast simple sequence repeat technique. We analyzed 11 polymorphic loci with clear and repeating bands, and we calculated the multi-locus outcrossing rate (tm), single-locus outcrossing rate, inbreeding index, and fixation index (F).

Optimization of chloroplast microsatellite PCR conditions and primer screening for endangered Rheum officinale, Rheum palmatum, and Rheum tanguticum

Y. Zhou, Guo, Z. J., Han, L., Li, Y., and Wang, X. M., Optimization of chloroplast microsatellite PCR conditions and primer screening for endangered Rheum officinale, Rheum palmatum, and Rheum tanguticum, vol. 13, pp. 5787-5794, 2014.

Chloroplast microsatellite primers were developed in order to provide more population genetic information of endangered Rheum officinale, R. palmatum, and R. tanguticum for conservation. The dried roots and rhizomes of these plants are important in traditional Chinese medicine. The results showed that the optimum concentrations of Mg2+, Taq DNA polymerase, dNTPs, template DNA, and primers in a 25-μL reaction system were 2.0 mM, 1.0 U, 0.10 mM, 20 ng, and 0.8 μM, respectively.

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