Chickpea

Genetic structure and diversity analysis of the primary gene pool of chickpea using SSR markers

P. Choudhary, Khanna, S. M., Jain, P. K., Bharadwaj, C., Kumar, J., Lakhera, P. C., and Srinivasan, R., “Genetic structure and diversity analysis of the primary gene pool of chickpea using SSR markers”, vol. 11, pp. 891-905, 2012.

Members of the primary gene pool of the chickpea, including 38 accessions of Cicer arietinum, six of C. reticulatum and four of C. echinospermum grown in India were investigated using 100 SSR markers to analyze their genetic structure, diversity and relationships. We found considerable diversity, with a mean of 4.8 alleles per locus (ranging from 2 to 11); polymorphic information content ranged from 0.040 to 0.803, with a mean of 0.536.

Determination of genetic variation among chickpea genotypes and their F1 crosses with RAPD markers

K. Mahmood, Nazeer, W., Naeem, M., Freed, S., Khan, M. A., Abbas, G., Iqbal, M., Kulachi, A. R., Sajid, S. A., Sadiq, M. A., and Saleem, M., “Determination of genetic variation among chickpea genotypes and their F1 crosses with RAPD markers”, vol. 12, pp. 2127-2137, 2013.

The intensity of genetic diversity amongst chickpea genotypes and their crosses is unknown. The current study investigated the genetic diversity of chickpea genotypes and their F1 crosses by using random amplified polymorphic DNA (RAPD) markers. We assessed the variation among six chickpea genotypes and 15 F1 crosses with the RAPD markers. The six parents and their 21 hybrids were carefully studied based on the presence or absence of bands. The level of polymorphism varied with different primers.

Genetic diversity of chickpea (Cicer arietinum L.) germplasm in Pakistan as revealed by RAPD analysis

F. Ahmad, Khan, A. I., Awan, F. S., Sadia, B., Sadaqat, H. A., and Bahadur, S., “Genetic diversity of chickpea (Cicer arietinum L.) germplasm in Pakistan as revealed by RAPD analysis”, vol. 9, pp. 1414-1420, 2010.

Genetic diversity analysis of chickpea germplasm can provide practical information for the selection of parental material and thus assist in planning breeding strategies. Chickpea seed is a good source of carbohydrates and proteins, constituting 80% of the total dry seed weight. Released cultivars and advanced lines of 30 chickpea genotypes were subjected to RAPD analysis for assessment of genetic diversity. We used 16 RAPD primers. Amplification of genomic DNA of the 30 genotypes yielded 62 fragments that could be scored.

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